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Detecting biochemical evidence for life with the signs of life detector (solid) in an anaerobic microorganism under fossilization conditions

Gomez, Felipe and Garda-Descalzo, Laura and Gayober, Frederic and Westall, Frances and Bohmeier, Maria and Schwendner, Petra and Cockell, Charles S. and the MASE team (2016) Detecting biochemical evidence for life with the signs of life detector (solid) in an anaerobic microorganism under fossilization conditions. 16th EANA Astrobiology Conference, 27. - 30. September 2016, Athens, Greece.

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Abstract

The definitive detection of biosignatures in the context of astrobiological missions to Mars is not without difficulty. Could it be possible to detect biomarkers from an extinct form of life in a very ancient material? The traces of some microorganisms can be well preserved thanks to rapid mineralization of the organisms and cementation of the sediments in which they occur [1]. Thus biosignatures could be indicators of either extant or extinct life, the search for which is one of the main objectives of Mars exploration [1]. The central motivation of the MASE project (Mars Analogues for Space Exploration) is to gain knowledge about the habitability of Mars by the study of the adaptation of anaerobic life forms to extreme environments, their environmental context, and the methods used to detect their biosignatures. Within this background a fundamental target of MASE project is to improve and optimize methods for biosignature detection in samples with low biomass from certain Mars analogue sites. In this context we applied antibody multiarray competitive immunoassay to follow the evolution of specific biochemical signatures from a culture under fossilization conditions. An antibody multiarray competitive immunoassay for the simultaneous detection of compounds of a wide range of molecular sizes or whole spores and cells [2] [3] has revealed as suitable option to achieve this MASE purpose. It consists in a rapid strategy to detect a huge set of different epitopes in extracted samples by a sandwich multiarray immunoassay in a slide or LDChip (Life Detector Chip) where huge range of different antibodies are coated. In this report, we present the results from an experiment in which we followed the biochemical signatures from a growing culture of an isolate of Yersinia sp. in fresh media and in a culture growing under fossilization conditions in silica and gypsum. A decrease in the signal of relative fluorescence of antibody-antigen binding (biomarkers detected) is observed when comparing an untreated Yersinia sp. culture and those induced to mineralization at different time points.

Item URL in elib:https://elib.dlr.de/107925/
Document Type:Conference or Workshop Item (Speech)
Title:Detecting biochemical evidence for life with the signs of life detector (solid) in an anaerobic microorganism under fossilization conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Gomez, FelipeCentro de Astrobiologia (CSIC-INTA) Carretera de Ajalvir, km 4 Torrejon de Ardoz 28850 Madrid; gomezgf (at) eab.inta-csie.esUNSPECIFIED
Garda-Descalzo, LauraCentro de Astrobiologia (CSIC-INTA) Carretera de Ajalvir, km 4 Torrejon de Ardoz 28850 MadridUNSPECIFIED
Gayober, FredericCNRS – Centre de Biophysique Moleculaire (UPR4301). Rue Charles Sadron CS80054 45071 Orleans Cedex 2UNSPECIFIED
Westall, FrancesCNRS – Centre de Biophysique Moleculaire (UPR4301). Rue Charles Sadron CS80054 45071 Orleans Cedex 2UNSPECIFIED
Bohmeier, MariaDeutsches Zentrum für Luft- und Raumfahrt e.V. (DLR) German Aerospace Center. Institute of Aerospace Medicine / Radiation Biology Department 1 Research Group Astrobiology. Linder Hoehe, 51147 Cologne, GermanyUNSPECIFIED
Schwendner, PetraSchool of Physics and Astronomy. University of Edinburgh, James Clerk Maxwell Building, Peter Guthrie Tait Roas, EdinburghUNSPECIFIED
Cockell, Charles S. and the MASE teamSchool of Physics and Astronomy. University of Edinburgh, James Clerk Maxwell Building, Peter Guthrie Tait Roas, EdinburghUNSPECIFIED
Date:2016
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Astrobiology, Mars, anaerobic microorganism, fossilization
Event Title:16th EANA Astrobiology Conference
Event Location:Athens, Greece
Event Type:international Conference
Event Dates:27. - 30. September 2016
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:25 Nov 2016 13:16
Last Modified:31 Jul 2019 20:05

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