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Cytosolic Calcium Concentration Changes in Neuronal Cells Under Clinorotation and in Parabolic Flight Missions

Hauslage, Jens and Abbrecht, Medea and Hanke, Lars and Hemmersbach, Ruth and Koch, Claudia and Hanke, Wolfgang and Kohn, Florian P. (2016) Cytosolic Calcium Concentration Changes in Neuronal Cells Under Clinorotation and in Parabolic Flight Missions. Microgravity Science and Technology. Springer. DOI: 10.1007/s12217-016-9520-y ISSN 0938-0108

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Official URL: http://link.springer.com/article/10.1007/s12217-016-9520-y

Abstract

All life on earth has been established under conditions of stable gravity of 1g. Nevertheless, in numerous experiments the direct gravity dependence of biological processes has been shown on all levels of organization, from single molecules to humans. To study the effects especially of microgravity on biological systems, a variety of platforms are available, from drop towers to the ISS. Due to the costs of these platforms and their limited availability, as an alternative, numerous simulators have been developed for so called “simulated” microgravity. A classical system is a clinostat, basically rotating a sample around one axis, and by integration of the gravity vector for 360 degree arguing that thus the effects of gravity are depleted. Indeed, a variety of studies has shown that taking out the direction of gravity from a biological system often results in consequences similar to the exposure of the system to real microgravity. Nevertheless, the opposite has been shown, too, and as a consequence the relevance of clinostats in microgravity research is still under discussion. To get some more insight into this problem we have constructed a small fluorescence clinostat and have studied the effects of clinorotation on the cytosolic calcium concentration of neuroglioma cells. The results have been compared to experiments with identical cells in real microgravity, utilizing parabolic flight missions. Our results show that in case of a cell suspension used in a small florescence clinostat within a tube diameter of 2mm, the effects of clinorotation are comparable to those under real microgravity, both showing a significant increase in intracellular calcium concentration.

Item URL in elib:https://elib.dlr.de/107036/
Document Type:Article
Title:Cytosolic Calcium Concentration Changes in Neuronal Cells Under Clinorotation and in Parabolic Flight Missions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hauslage, JensGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Abbrecht, MedeaUniversity of Hohenheim, Institute of Physiology, Stuttgart, GermanyUNSPECIFIED
Hanke, LarsGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Koch, ClaudiaUniversity of Hohenheim, Institute of Physiology, Stuttgart, GermanyUNSPECIFIED
Hanke, WolfgangUniversity of Hohenheim, Institute of Physiology, Stuttgart, GermanyUNSPECIFIED
Kohn, Florian P.University of Hohenheim, Institute of Physiology, Stuttgart, GermanyUNSPECIFIED
Date:26 September 2016
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-016-9520-y
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Clinostat, Simulated microgravity, Neuroglioma cells, Cytosolic calcium concentration, Fluorescence, Parabolic flights
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Project Mapheus (payload)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:25 Nov 2016 13:14
Last Modified:25 Nov 2016 13:14

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