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Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing

Nagler, Katja and Krawczyk, Antonina O. and De Jong, Anne and Madela, Kazimierz and Hoffmann, Tamara and Laue, Michael and Kuipers, Oscar P. and Bremer, Erhard and Moeller, Ralf (2016) Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing. Frontiers in Microbiology, 7. Frontiers Media S.A.. DOI: 10.3389/fmicb.2016.01564 ISSN 1664-302X

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Official URL: http://dx.doi.org/10.3389/fmicb.2016.01564

Abstract

In its natural habitat, the soil bacterium Bacillus subtilis often has to cope with fluctuating osmolality and nutrient availability. Upon nutrient depletion it can form dormant spores, which can revive to form vegetative cells when nutrients become available again. While the effects of salt stress on spore germination have been analyzed previously, detailed knowledge on the salt stress response during the subsequent outgrowth phase is lacking. In this study, we investigated the changes in gene expression during B. subtilis outgrowth in the presence of 1.2 M NaCl using RNA sequencing. In total, 402 different genes were upregulated and 632 genes were downregulated during 90 min of outgrowth in the presence of salt. The salt stress response of outgrowing spores largely resembled the osmospecific response of vegetative cells exposed to sustained high salinity and included strong upregulation of genes involved in osmoprotectant uptake and compatible solute synthesis. The σB-dependent general stress response typically triggered by salt shocks was not induced, whereas the σW regulon appears to play an important role for osmoadaptation of outgrowing spores. Furthermore, high salinity induced many changes in the membrane protein and transporter transcriptome. Overall, salt stress seemed to slow down the complex molecular reorganization processes (“ripening”) of outgrowing spores by exerting detrimental effects on vegetative functions such as amino acid metabolism.

Item URL in elib:https://elib.dlr.de/106694/
Document Type:Article
Title:Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Nagler, KatjaSpace Microbiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center Cologne, GermanyUNSPECIFIED
Krawczyk, Antonina O.Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsUNSPECIFIED
De Jong, AnneDepartment of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsUNSPECIFIED
Madela, KazimierzAdvanced Light and Electron Microscopy, Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, GermanyUNSPECIFIED
Hoffmann, TamaraLaboratory of Microbiology, Department of Biology, Philipps-University Marburg, Marburg, GermanyUNSPECIFIED
Laue, MichaelAdvanced Light and Electron Microscopy, Center for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, GermanyUNSPECIFIED
Kuipers, Oscar P.Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, NetherlandsUNSPECIFIED
Bremer, ErhardLaboratory of Microbiology, Department of Biology, Philipps-University Marburg, Marburg, GermanyUNSPECIFIED
Moeller, RalfSpace Microbiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center Cologne, Germany; ralf.moeller (at) dlr.deUNSPECIFIED
Date:2016
Journal or Publication Title:Frontiers in Microbiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:7
DOI :10.3389/fmicb.2016.01564
Publisher:Frontiers Media S.A.
ISSN:1664-302X
Status:Published
Keywords:B.subtilis, spore germination, outgrowth, ripening, high salinity,osmotic stress, NaCl, RNA-seq
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:25 Nov 2016 13:13
Last Modified:25 Nov 2016 13:13

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