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Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance

Beblo-Vranesevic, Kristina and Koschnitzki, Dagmar and Rettberg, Petra (2016) Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance. DGDR 2016, 12.-16. September 2016, Essen, Germany.

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Abstract

Ignicoccus hospitalis´ tolerance to ionizing and non-ionizing radiation has been studied extensively, and the DNA damages were visualized on a molecular level. Ignicoccus species, strictly anaerobic, hyperthermophilic (Topt. 90°C), chemolithoautotrophic, were found in deep-sea hydrothermal vents, and belong to the phylogenetically deep-branching crenarchaeal phylum. In this study I. hospitalis has been characterized in respect to its tolerance towards non-ionizing and ionizing radiation. High fluences (1000 J/m²) of monochromatic UV-C (254 nm) led to a reduction in survivability of 2 to 3 orders of magnitude. Focusing on ionizing radiation, I. hospitalis showed a strong X-ray tolerance, although never exposed to it in the natural habitat. Cells survived an exposure with up to 24 kGy. This tolerance gives first hints on highly effective DNA repair mechanisms for maintaining DNA integrity in life-hostile terrestrial environments. Up to now, little is known of the repair genes and mechanisms which are active in I. hospitalis. DNA damages caused by X-ray irradiation were analyzed using agarose gel electrophoresis: increasing X-ray doses led to increased DNA damages in terms of strand breaks. PCR-based assays like RAPD analysis (randomly amplified polymorphic DNA) qualitatively confirmed changes in the genomic DNA after irradiation treatment. Irradiated cells showed different band patterns compared to non-treated cells. Future experiments will focus to uncover unknown genes involved in DNA repair and their underlying repair mechanism. In addition, it will be interesting to investigate whether repair mechanisms involved in the repair of damages induced by radiation are also used for repairing DNA damage caused by other environmental factors.

Item URL in elib:https://elib.dlr.de/106179/
Document Type:Conference or Workshop Item (Speech)
Title:Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Beblo-Vranesevic, KristinaDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121
Koschnitzki, DagmarDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace CenterUNSPECIFIED
Rettberg, PetraDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Centerhttps://orcid.org/0000-0003-4439-2395
Date:2016
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Ignicoccus hospitalis, ionizing and non-ionizing Radiation, DNA damage, DNA repair
Event Title:DGDR 2016
Event Location:Essen, Germany
Event Type:international Conference
Event Dates:12.-16. September 2016
Organizer:Deutsche Gesellschaft für DNA-Reparaturforschung
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:10 Oct 2016 15:50
Last Modified:31 Jul 2019 20:03

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