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Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance

Beblo-Vranesevic, Kristina und Koschnitzki, Dagmar und Rettberg, Petra (2016) Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance. DGDR 2016, 2016-09-12 - 2016-09-16, Essen, Germany.

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Kurzfassung

Ignicoccus hospitalis´ tolerance to ionizing and non-ionizing radiation has been studied extensively, and the DNA damages were visualized on a molecular level. Ignicoccus species, strictly anaerobic, hyperthermophilic (Topt. 90°C), chemolithoautotrophic, were found in deep-sea hydrothermal vents, and belong to the phylogenetically deep-branching crenarchaeal phylum. In this study I. hospitalis has been characterized in respect to its tolerance towards non-ionizing and ionizing radiation. High fluences (1000 J/m²) of monochromatic UV-C (254 nm) led to a reduction in survivability of 2 to 3 orders of magnitude. Focusing on ionizing radiation, I. hospitalis showed a strong X-ray tolerance, although never exposed to it in the natural habitat. Cells survived an exposure with up to 24 kGy. This tolerance gives first hints on highly effective DNA repair mechanisms for maintaining DNA integrity in life-hostile terrestrial environments. Up to now, little is known of the repair genes and mechanisms which are active in I. hospitalis. DNA damages caused by X-ray irradiation were analyzed using agarose gel electrophoresis: increasing X-ray doses led to increased DNA damages in terms of strand breaks. PCR-based assays like RAPD analysis (randomly amplified polymorphic DNA) qualitatively confirmed changes in the genomic DNA after irradiation treatment. Irradiated cells showed different band patterns compared to non-treated cells. Future experiments will focus to uncover unknown genes involved in DNA repair and their underlying repair mechanism. In addition, it will be interesting to investigate whether repair mechanisms involved in the repair of damages induced by radiation are also used for repairing DNA damage caused by other environmental factors.

elib-URL des Eintrags:https://elib.dlr.de/106179/
Dokumentart:Konferenzbeitrag (Vortrag)
Titel:Ignicoccus hospitalis: a polyextremophilic microorganism with unusual high radiation tolerance
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Beblo-Vranesevic, KristinaDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121NICHT SPEZIFIZIERT
Koschnitzki, DagmarDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace CenterNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Rettberg, PetraDepartment of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Centerhttps://orcid.org/0000-0003-4439-2395NICHT SPEZIFIZIERT
Datum:2016
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:Ignicoccus hospitalis, ionizing and non-ionizing Radiation, DNA damage, DNA repair
Veranstaltungstitel:DGDR 2016
Veranstaltungsort:Essen, Germany
Veranstaltungsart:internationale Konferenz
Veranstaltungsbeginn:12 September 2016
Veranstaltungsende:16 September 2016
Veranstalter :Deutsche Gesellschaft für DNA-Reparaturforschung
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:10 Okt 2016 15:50
Letzte Änderung:24 Apr 2024 20:11

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