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Germination of Spores of Astrobiologically Relevant Bacillus Species in High-Salinity Environments

Nagler, Katja and Julius, Christina and Moeller, Ralf (2016) Germination of Spores of Astrobiologically Relevant Bacillus Species in High-Salinity Environments. Astrobiology, 16 (7). Mary Ann Liebert Inc.. DOI: 10.1089/ast.2015.1419 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2015.1419

Abstract

In times of increasing space exploration and search for extraterrestrial life, new questions and challenges for planetary protection, aiming to avoid forward contamination of different planets or moons with terrestrial life, are emerging. Spore-forming bacteria such as Bacillus species have a high contamination potential due to their spores’ extreme resistance, enabling them to withstand space conditions. Spores require liquid water for their conversion into a growing cell (i.e., spore germination and subsequent growth). If present, water on extraterrestrial planets or moons is likely to be closely associated with salts (e.g., in salty oceans or brines), thus constituting high-salinity environments. Spores of Bacillus subtilis can germinate despite very high salt concentrations, although salt stress does exert negative effects on this process. In this study, germination and metabolic reactivation (‘‘outgrowth’’) of spores of five astrobiologically relevant Bacillus species (B. megaterium, B. pumilus SAFR-032, B. nealsonii, B. mojavensis, and B. vallismortis) in high salinity (≤3.6 M NaCl) were investigated. Spores of different species exhibited different germination and outgrowth capabilities in high salinity, which strongly depended on germination conditions, especially the exact composition of the medium. In this context, a new ‘‘universal’’ germination trigger for Bacillus spores, named KAGE (KCl, L-alanine, D-glucose, ectoine), was identified, which will be very useful for future comparative germination and outgrowth studies on different Bacillus species. Overall, this study yielded interesting new insights on salt stress effects on spore germination and points out the difficulty of predicting the potential of spores to contaminate salty environments on extraterrestrial celestial bodies.

Item URL in elib:https://elib.dlr.de/104792/
Document Type:Article
Title:Germination of Spores of Astrobiologically Relevant Bacillus Species in High-Salinity Environments
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Nagler, KatjaGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), GermanyUNSPECIFIED
Julius, ChristinaGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), GermanyUNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne (Köln), Germany; E-mail: ralf.moeller (at) dlr.deUNSPECIFIED
Date:2016
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:16
DOI :10.1089/ast.2015.1419
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Bacillus species, Spores, Germination, High salinity, Salt stress, NaCl, Inhibition
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:22 Jun 2016 12:01
Last Modified:06 Sep 2019 15:23

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