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Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization

Raguse, Marina and Fiebrandt, Marcel and Denis, Benjamin and Stapelmann, Katharina and Eichenberger, Patrick and Driks, Adam and Eaton, Peter and Awakowicz, Peter and Moeller, Ralf (2016) Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization. Journal of Physics D: Applied Physics, 49 (28), p. 285401. Institute of Physics (IOP) Publishing. DOI: 10.1088/0022-3727/49/28/285401 ISSN 0022-3727

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Official URL: http://dx.doi.org/10.1088/0022-3727/49/28/285401

Abstract

Low-pressure plasmas have been evaluated for their potential in biomedical and defense purposes. The sterilizing effect of plasma can be attributed to several active agents, including (V)UV radiation, charged particles, radical species, neutral and excited atoms and molecules, and the electric field. Spores of Bacillus subtilis were used as a bioindicator and a genetic model system to study the sporicidal effects of low-pressure plasma decontamination. Wild-type spores, spores lacking the major protective coat layers (inner, outer, and crust), pigmentation-deficient spores or spore impaired in encasement (a late step in coat assembly) were systematically tested for their resistance to low-pressure argon, hydrogen, and oxygen plasmas with and without admixtures. We demonstrate that low-pressure plasma discharges of argon and oxygen discharges cause significant physical damage to spore surface structures as visualized by atomic force microscopy. Spore resistance to low-pressure plasma was primarily dependent on the presence of the inner, and outer spore coat layers as well as spore encasement, with minor or less importance of the crust and spore pigmentation, whereas spore inactivation itself was strongly influenced by the gas composition and operational settings.

Item URL in elib:https://elib.dlr.de/104697/
Document Type:Article
Title:Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Raguse, MarinaGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Linder Höhe, Cologne (Köln), GermanyUNSPECIFIED
Fiebrandt, MarcelRuhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, GermanyUNSPECIFIED
Denis, BenjaminRuhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, GermanyUNSPECIFIED
Stapelmann, KatharinaRuhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, GermanyUNSPECIFIED
Eichenberger, PatrickCenter for Genomics and Systems Biology, Department of Biology, New York University, New York, NY, USAUNSPECIFIED
Driks, AdamDepartment of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL, USAUNSPECIFIED
Eaton, PeterUCIBIO/Requimte, Department of Chemistry and Biochemistry, Faculty of Sciences of the University of Porto, Porto, PortugalUNSPECIFIED
Awakowicz, PeterRuhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, GermanyUNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Linder Höhe, Cologne (Köln), GermanyUNSPECIFIED
Date:2016
Journal or Publication Title:Journal of Physics D: Applied Physics
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:49
DOI :10.1088/0022-3727/49/28/285401
Page Range:p. 285401
Publisher:Institute of Physics (IOP) Publishing
ISSN:0022-3727
Status:Published
Keywords:bioindicator, plasma sterilization, decontamination, spores, Bacillus subtilis, spore coat, spore resistance
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:22 Jun 2016 12:01
Last Modified:08 Mar 2018 18:37

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