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Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments

Tauber, Svantje and Hauschild, Swantje and Paulsen, Katrin and Gutewort, Annett and Raig, Christiane and Hürlimann, Eva and Biskup, Josefine and Philpot, Claudia and Lier, Hartwin and Engelmann, Frank and Pantaleo, Antonella and Cogoli, Augusto and Pippia, Proto and Layer, Liliana E. and Thiel, Cora S. and Ullrich, Oliver (2015) Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments. Cellular Physiology and Biochemistry, p. 10341051. S. Karger AG. doi: 10.1159/000373930. ISSN 1015-8987.

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Official URL: https://www.karger.com/Article/FullText/373930

Abstract

Background/Aims: Several limiting factors for human health and performance in microgravity have been clearly identified arising from the immune system, and substantial research activities are required in order to provide the basic information for appropriate integrated risk management. The gravity-sensitive nature of cells of the immune system renders them an ideal biological model in search for general gravity-sensitive mechanisms and to understand how the architecture and function of human cells is related to the gravitational force and therefore adapted to life on Earth. Methods: We investigated the influence of altered gravity in parabolic flight and 2D clinostat experiments on key proteins of activation and signaling in primary T lymphocytes. We quantified components of the signaling cascade 1.) in nonactivated T lymphocytes to assess the “basal status” of the cascade and 2.) in the process of activation to assess the signal transduction. Results: We found a rapid decrease of CD3 and IL-2R surface expression and reduced p-LAT after 20 seconds of altered gravity in nonactivated primary T lymphocytes during parabolic flight. Furthermore, we observed decreased CD3 surface expression, reduced ZAP-70 abundance and increased histone H3-acetylation in activated T lymphocytes after 5 minutes of clinorotation and a transient downregulation of CD3 and stable downregulation of IL-2R during 60 minutes of clinorotation. Conclusion: CD3 and IL-2R are downregulated in primary T lymphocytes in altered gravity. We assume that a gravity condition around 1g is required for the expression of key surface receptors and appropriate regulation of signal molecules in T lymphocytes.

Item URL in elib:https://elib.dlr.de/104290/
Document Type:Article
Additional Information:Clinosat / 19. Parabelflugkampagne
Title:Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Tauber, SvantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Hauschild, SwantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Paulsen, KatrinUniversity of Zurich, Faculty of MedicineUNSPECIFIEDUNSPECIFIED
Gutewort, AnnettInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Raig, ChristianeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Hürlimann, EvaAnatomisches Institut, Universität Zürich, Winterthurer Str. 190, CH-8057 Zürich, SchweizUNSPECIFIEDUNSPECIFIED
Biskup, JosefineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Philpot, ClaudiaDLRUNSPECIFIEDUNSPECIFIED
Lier, HartwinKEK GmbHUNSPECIFIEDUNSPECIFIED
Engelmann, FrankUniversity of Applied Sciences JenaUNSPECIFIEDUNSPECIFIED
Pantaleo, AntonellaUniversity of Sassari, Dpt of Biomedical SciencesUNSPECIFIEDUNSPECIFIED
Cogoli, AugustoETH Zürich / BIOTESCUNSPECIFIEDUNSPECIFIED
Pippia, ProtoUniversity of Sassari, Dpt of Biomedical SciencesUNSPECIFIEDUNSPECIFIED
Layer, Liliana E.University of Zurich, Faculty of MedicineUNSPECIFIEDUNSPECIFIED
Thiel, Cora S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ullrich, OliverUniversity of Zurich, Institute of Anatomy, Zurich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Date:2 February 2015
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI:10.1159/000373930
Page Range:p. 10341051
Publisher:S. Karger AG
ISSN:1015-8987
Status:Published
Keywords:T cell activation• Microgravity • Gravi-sensitivity • Hypergravity • Parabolic flight • Clinorotation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space System Technology
DLR - Research area:Raumfahrt
DLR - Program:R SY - Space System Technology
DLR - Research theme (Project):R - Systemanalyse Raumsegment (old)
Location: Bremen
Institutes and Institutions:Institute of Space Systems > System Analysis Space Segment
Deposited By: Philpot, Dipl.-Ing. Claudia
Deposited On:17 May 2016 09:46
Last Modified:20 Jun 2023 04:13

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