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Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments

Tauber, Svantje and Hauschild, Swantje and Paulsen, Katrin and Gutewort, Annett and Raig, Christiane and Hürlimann, Eva and Biskup, Josefine and Philpot, Claudia and Lier, Hartwin and Engelmann, Frank and Pantaleo, Antonella and Cogoli, Augusto and Pippia, Proto and Layer, Liliana E. and Thiel, Cora S. and Ullrich, Oliver (2015) Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments. Cellular Physiology and Biochemistry, p. 10341051. S. Karger AG. DOI: 10.1159/000373930 ISSN 1015-8987

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Official URL: https://www.karger.com/Article/FullText/373930

Abstract

Background/Aims: Several limiting factors for human health and performance in microgravity have been clearly identified arising from the immune system, and substantial research activities are required in order to provide the basic information for appropriate integrated risk management. The gravity-sensitive nature of cells of the immune system renders them an ideal biological model in search for general gravity-sensitive mechanisms and to understand how the architecture and function of human cells is related to the gravitational force and therefore adapted to life on Earth. Methods: We investigated the influence of altered gravity in parabolic flight and 2D clinostat experiments on key proteins of activation and signaling in primary T lymphocytes. We quantified components of the signaling cascade 1.) in nonactivated T lymphocytes to assess the “basal status” of the cascade and 2.) in the process of activation to assess the signal transduction. Results: We found a rapid decrease of CD3 and IL-2R surface expression and reduced p-LAT after 20 seconds of altered gravity in nonactivated primary T lymphocytes during parabolic flight. Furthermore, we observed decreased CD3 surface expression, reduced ZAP-70 abundance and increased histone H3-acetylation in activated T lymphocytes after 5 minutes of clinorotation and a transient downregulation of CD3 and stable downregulation of IL-2R during 60 minutes of clinorotation. Conclusion: CD3 and IL-2R are downregulated in primary T lymphocytes in altered gravity. We assume that a gravity condition around 1g is required for the expression of key surface receptors and appropriate regulation of signal molecules in T lymphocytes.

Item URL in elib:https://elib.dlr.de/104290/
Document Type:Article
Additional Information:Clinosat / 19. Parabelflugkampagne
Title:Signal Transduction in Primary Human T Lymphocytes in Altered Gravity During Parabolic Flight and Clinostat Experiments
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Tauber, SvantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Hauschild, SwantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Paulsen, KatrinUniversity of Zurich, Faculty of MedicineUNSPECIFIED
Gutewort, AnnettInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Raig, ChristianeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Hürlimann, EvaAnatomisches Institut, Universität Zürich, Winterthurer Str. 190, CH-8057 Zürich, SchweizUNSPECIFIED
Biskup, JosefineInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; josefine.biskup (at) hotmail.comUNSPECIFIED
Philpot, ClaudiaDLRUNSPECIFIED
Lier, HartwinKEK GmbHUNSPECIFIED
Engelmann, FrankUniversity of Applied Sciences JenaUNSPECIFIED
Pantaleo, AntonellaUniversity of Sassari, Dpt of Biomedical SciencesUNSPECIFIED
Cogoli, AugustoETH Zürich / BIOTESCUNSPECIFIED
Pippia, ProtoUniversity of Sassari, Dpt of Biomedical SciencesUNSPECIFIED
Layer, Liliana E.University of Zurich, Faculty of MedicineUNSPECIFIED
Thiel, Cora S.Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland; cora.thiel (at) uzh.chUNSPECIFIED
Ullrich, OliverUniversity of Zurich, Institute of Anatomy, Zurich, SwitzerlandUNSPECIFIED
Date:2 February 2015
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
Open Access:Yes
In DOAJ:Yes
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1159/000373930
Page Range:p. 10341051
Publisher:S. Karger AG
ISSN:1015-8987
Status:Published
Keywords:T cell activation• Microgravity • Gravi-sensitivity • Hypergravity • Parabolic flight • Clinorotation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Technology
DLR - Research area:Raumfahrt
DLR - Program:R SY - Technik für Raumfahrtsysteme
DLR - Research theme (Project):R - Systemanalyse Raumsegment
Location: Bremen
Institutes and Institutions:Institute of Space Systems > Systemanalyse Raumsegmente
Deposited By: Philpot, Dipl.-Ing. Claudia
Deposited On:17 May 2016 09:46
Last Modified:23 Jan 2019 05:02

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