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Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

Thiel, Cora S. and Hauschild, Swantje and Tauber, Svantje and Paulsen, Katrin and Raig, Christiane and Raem, Arnold and Biskup, Josefine and Gutewort, Annett and Hürlimann, Eva and Unverdorben, Felix and Buttron, Isabell and Lauber, Beatrice and Philpot, Claudia and Lier, Hartwin and Engelmann, Frank and Layer, Liliana E. and Ullrich, Oliver (2015) Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity. BioMed Research International, pp. 1-20. Hindawi Publishing Corporation. DOI: 10.1155/2015/363575 ISBN 2314-6133 ISSN 2314-6133

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Official URL: http://www.zora.uzh.ch/106978/

Abstract

Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms. For the process of normalization, reference genes (“housekeeping genes”) are essential to verify gene expression analysis. Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions.However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet. The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states. In our study, we searched for potential reference genes with stable expression during different gravitational conditions (microgravity, normogravity, and hypergravity) which are additionally not altered in different hardware systems. We were able to identify eight genes (ALB, B4GALT6, GAPDH, HMBS, YWHAZ, ABCA5, ABCA9, and ABCC1) which demonstrated no altered gene expression levels in all tested conditions and therefore represent good candidates for the standardization of gene expression studies in altered gravity.

Item URL in elib:https://elib.dlr.de/104289/
Document Type:Article
Title:Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Thiel, Cora S.Institute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland; cora.thiel (at) uzh.chUNSPECIFIED
Hauschild, SwantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Zurich, Switzerland; cora.thiel (at) uzh.chUNSPECIFIED
Tauber, SvantjeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Paulsen, KatrinUniversity of Zurich, Institute of Anatomy, Zurich, SwitzerlandUNSPECIFIED
Raig, ChristianeInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Raem, ArnoldArrows Biomedical Deutschland GmbHUNSPECIFIED
Biskup, JosefineInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; josefine.biskup (at) hotmail.comUNSPECIFIED
Gutewort, AnnettInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Hürlimann, EvaAnatomisches Institut, Universität Zürich, Winterthurer Str. 190, CH-8057 Zürich, SchweizUNSPECIFIED
Unverdorben, FelixInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Buttron, IsabellInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Lauber, BeatriceInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Philpot, ClaudiaDLRUNSPECIFIED
Lier, HartwinKEK GmbHUNSPECIFIED
Engelmann, FrankUniversity of Applied Sciences JenaUNSPECIFIED
Layer, Liliana E.Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, SwitzerlandUNSPECIFIED
Ullrich, OliverUniversity of Zurich, Institute of Anatomy, Zurich, SwitzerlandUNSPECIFIED
Date:13 January 2015
Journal or Publication Title:BioMed Research International
Refereed publication:Yes
Open Access:Yes
In DOAJ:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1155/2015/363575
Page Range:pp. 1-20
Publisher:Hindawi Publishing Corporation
ISSN:2314-6133
ISBN:2314-6133
Status:Published
Keywords:Gene expression
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Technology
DLR - Research area:Raumfahrt
DLR - Program:R SY - Technik für Raumfahrtsysteme
DLR - Research theme (Project):R - Systemanalyse Raumsegment
Location: Bremen
Institutes and Institutions:Institute of Space Systems > Systemanalyse Raumsegmente
Deposited By: Philpot, Dipl.-Ing. Claudia
Deposited On:17 May 2016 09:44
Last Modified:08 Mar 2018 18:36

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