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Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies

Raguse, Marina and Fiebrandt, Marcel and Stapelmann, Katharina and Madela, Kazimierz and Laue, Michael and Lackmann, Jan-Wilm and Thwaite, Joanne E. and Setlow, Peter and Awakowicz, Peter and Moeller, Ralf and Schaffner, D. W. (2016) Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies. Applied and Environmental Microbiology, 82 (7), pp. 2031-2038. American Society for Microbiology. DOI: 10.1128/AEM.03934-15 ISSN 0099-2240

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Official URL: http://dx.doi.org/10.1128/AEM.03934-15

Abstract

Novel decontamination technologies, including cold low-pressure plasma and blue light (400 nm), are promising alternatives to conventional surface decontamination methods. However, the standardization of the assessment of such sterilization processes remains to be accomplished. Bacterial endospores of the genera Bacillus and Geobacillus are frequently used as biological indicators (BIs) of sterility. Ensuring standardized and reproducible BIs for reliable testing procedures is a significant problem in industrial settings. In this study, an electrically driven spray deposition device was developed, allowing fast, reproducible, and homogeneous preparation of Bacillus subtilis 168 spore monolayers on glass surfaces. A detailed description of the structural design as well as the operating principle of the spraying device is given. The reproducible formation of spore monolayers of up to 5x10⁷ spores per sample was verified by scanning electron microscopy. Surface inactivation studies revealed that monolayered spores were inactivated by UV-C (254 nm), low-pressure argon plasma (500 W, 10 Pa, 100 standard cubic cm per min), and blue light (400 nm) significantly faster than multilayered spores were. We have thus succeeded in the uniform preparation of reproducible, highly concentrated spore monolayers with the potential to generate BIs for a variety of nonpenetrating surface decontamination techniques.

Item URL in elib:https://elib.dlr.de/103592/
Document Type:Article
Title:Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Raguse, MarinaGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne, GermanyUNSPECIFIED
Fiebrandt, MarcelRuhr University Bochum, Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Bochum, GermanyUNSPECIFIED
Stapelmann, KatharinaRuhr University Bochum, Institute of Electrical Engineering and Plasma Technology, Biomedical Applications of Plasma Technology, Bochum, GermanyUNSPECIFIED
Madela, KazimierzRobert Koch Institute Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Laue, MichaelRobert Koch Institute Advanced Light and Electron Microscopy, Berlin, GermanyUNSPECIFIED
Lackmann, Jan-WilmRuhr University Bochum, Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Bochum, Germany and Ruhr University Bochum, Institute of Electrical Engineering and Plasma Technology, BiomedUNSPECIFIED
Thwaite, Joanne E.Defence Science and Technology Laboratory, Chemical, Biological and Radiological Division, Salisbury, United KingdomUNSPECIFIED
Setlow, PeterUniversity of Connecticut Health Center, Department of Molecular Biology and Biophysics, Farmington, Connecticut, USAUNSPECIFIED
Awakowicz, PeterRuhr University Bochum, Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Bochum, GermanyUNSPECIFIED
Moeller, RalfGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Space Microbiology Research Group, Cologne, GermanyUNSPECIFIED
Schaffner, D. W.EditorUNSPECIFIED
Date:2016
Journal or Publication Title:Applied and Environmental Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:82
DOI :10.1128/AEM.03934-15
Page Range:pp. 2031-2038
Publisher:American Society for Microbiology
ISSN:0099-2240
Status:Published
Keywords:enhanced spore decontamination technologies, Bacillus subtilis, spore monolayers
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:23 Mar 2016 10:50
Last Modified:10 May 2016 23:46

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