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Pipette-based Method to Study Embryoid Body Formation Derived from Mouse and Human Pluripotent Stem Cells Partially Recapitulating Early Embryonic Development Under Simulated Microgravity Conditions

Shinde, Vaibhav and Brungs, Sonja and Hescheler, Jürgen and Hemmersbach, Ruth and Sachinidis, Apapios (2015) Pipette-based Method to Study Embryoid Body Formation Derived from Mouse and Human Pluripotent Stem Cells Partially Recapitulating Early Embryonic Development Under Simulated Microgravity Conditions. Microgravity Science and Technology, pp. 1-9. Springer. DOI: 10.1007/s12217-015-9469-2 ISSN 0938-0108

Full text not available from this repository.

Abstract

The in vitro differentiation of pluripotent stem cells partially recapitulates early in vivo embryonic development. More recently, embryonic development under the influence of microgravity has become a primary focus of space life sciences. In order to integrate the technique of pluripotent stem cell differentiation with simulated microgravity approaches, the 2-D clinostat compatible pipette based method was experimentally investigated and adapted for investigating stem cell differentiation processes under simulated microgravity conditions. In order to keep residual accelerations as low as possible during clinorotation, while also guaranteeing enough material for further analysis, stem cells were exposed in 1-mL pipettes with a diameter of 3.5 mm. The differentiation of mouse and human pluripotent stem cells inside the pipettes resulted in the formation of embryoid bodies at normal gravity (1 g) after 24 h and 3 days. Differentiation of the mouse pluripotent stem cells on a 2-D pipette-clinostat for 3 days also resulted in the formation of embryoid bodies. Interestingly, the expression of myosin heavy chain was downregulated when cultivation was continued for an additional 7 days at normal gravity. This paper describes the techniques for culturing and differentiation of pluripotent stem cells and exposure to simulated microgravity during culturing or differentiation on a 2-D pipette clinostat. The implementation of these methodologies along with -omics technologies will contribute to understand the mechanisms regulating how microgravity influences early embryonic development.

Item URL in elib:https://elib.dlr.de/102920/
Document Type:Article
Title:Pipette-based Method to Study Embryoid Body Formation Derived from Mouse and Human Pluripotent Stem Cells Partially Recapitulating Early Embryonic Development Under Simulated Microgravity Conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Shinde, VaibhavUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Brungs, SonjaGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Hescheler, JürgenUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Sachinidis, ApapiosUniversity of Cologne, Institute of Neurophysiology and Center for Molecular Medicine Cologne (CMMC), Cologne, GermanyUNSPECIFIED
Date:5 November 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9469-2
Page Range:pp. 1-9
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:2-D clinostat, Embryonic stem cells, Induced pluripotent stem cells, Simulated microgravity
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:09 Feb 2016 12:30
Last Modified:10 May 2016 23:44

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