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ARADISH - Development of a Standardized Plant Growth Chamber for Experiments in Gravitational Biology Using Ground-Based Facilities

Schüler, Oliver and Krause, Lars and Görög, Mark and Hauslage, Jens and Kesseler, Leona and Böhmer, Maik and Hemmersbach, Ruth (2015) ARADISH - Development of a Standardized Plant Growth Chamber for Experiments in Gravitational Biology Using Ground-Based Facilities. Microgravity Science and Technology. Springer. DOI: 10.1007/s12217-015-9454-9 ISSN 0938-0108

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Official URL: http://dx.doi.org/10.1007/s12217-015-9454-9

Abstract

Plant development strongly relies on environ- mental conditions. Growth of plants in Biological Life Support Systems (BLSS), which are a necessity to allow human survival during long-term space exploration missions, poses a particular problem for plant growth, as in addition to the traditional environmental factors, microgravity (or reduced gravity such as on Moon or Mars) and limited gas exchange hamper plant growth. Studying the effects of reduced gravity on plants requires real or simulated microgravity experiments under highly standardized conditions, in order to avoid the influence of other environmental factors. Analysis of a large number of biological replicates, which is necessary for the detection of subtle phenotypical differences, can so far only be achieved in Ground-Based Facilities (GBF). Besides different experimental conditions, the usage of a variety of different plant growth chambers was a major factor that led to a lack of reproducibility and comparability in previous studies. We have developed a flexible and customizable plant growth chamber, called ARAbidopsis DISH (ARADISH), which allows plant growth from seed to seedling, being realized in a hydroponic system or on Agar. By developing a special holder, the ARADISH can be used for experiments with Arabidopsis thaliana or a plant with a similar habitus on common GBF hardware, including 2D clinostats and Random Positioning Machines (RPM). The ARADISH growth chamber has a controlled illumination system of red and blue light emitting diodes (LED), which allows the user to apply defined light conditions. As a proof of concept we tested a prototype in a proteomic experiment in which plants were exposed to simulated microgravity or a 90◦ stimulus. We optimized the design and performed viability tests after several days of growth in the hardware that underline the utility of ARADISH in microgravity research.

Item URL in elib:https://elib.dlr.de/102797/
Document Type:Article
Title:ARADISH - Development of a Standardized Plant Growth Chamber for Experiments in Gravitational Biology Using Ground-Based Facilities
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Schüler, OliverGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Krause, LarsGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Görög, MarkGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Hauslage, JensGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Kesseler, LeonaGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Böhmer, MaikWestfälische Wilhelms-Universität Münster, Institute for Biology and Biotechnology of Plants, Münster, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Date:3 September 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9454-9
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Ground-Based Facilities, Simulated microgravity, Biological Life Support Systems, Arabidopsis thaliana, Light Emitting Diodes (LED), Proteomics
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:03 Feb 2016 10:33
Last Modified:10 May 2016 23:44

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