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The influence of simulated microgravity on the proteome of Daphnia magna

Trotter, Benjamin and Otte, Kathrin A. and Schoppmann, Kathrin and Hemmersbach, Ruth and Fröhlich, Thomas and Arnold, Georg J. and Laforsch, Christian (2015) The influence of simulated microgravity on the proteome of Daphnia magna. npj Microgravity, 2015 (1), pp. 1-10. Nature Publishing Group. DOI: 10.1038/npjmgrav.2025.16 ISSN 2373-8065

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Official URL: http://www.nature.com/articles/npjmgrav201516

Abstract

BACKGROUND: The waterflea Daphnia is an interesting candidate for bioregenerative life support systems (BLSS). These animals are particularly promising because of their central role in the limnic food web and its mode of reproduction. However, the response of Daphnia to altered gravity conditions has to be investigated, especially on the molecular level, to evaluate the suitability of Daphnia for BLSS in space. METHODS: In this study, we applied a proteomic approach to identify key proteins and pathways involved in the response of Daphnia to simulated microgravity generated by a two-dimensional (2D) clinostat. We analyzed five biological replicates using 2D-difference gel electrophoresis proteomic analysis. RESULTS: We identified 109 protein spots differing in intensity (Po0.05). Substantial fractions of these proteins are involved in actin microfilament organization, indicating the disruption of cytoskeletal structures during clinorotation. Furthermore, proteins involved in protein folding were identified, suggesting altered gravity induced breakdown of protein structures in general. In addition, simulated microgravity increased the abundance of energy metabolism-related proteins, indicating an enhanced energy demand of Daphnia. CONCLUSIONS: The affected biological processes were also described in other studies using different organisms and systems either aiming to simulate microgravity conditions or providing real microgravity conditions. Moreover, most of the Daphnia protein sequences are well-conserved throughout taxa, indicating that the response to altered gravity conditions in Daphnia follows a general concept.

Item URL in elib:https://elib.dlr.de/102780/
Document Type:Article
Title:The influence of simulated microgravity on the proteome of Daphnia magna
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Trotter, BenjaminLaboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University Munich, Munich, GermanyUNSPECIFIED
Otte, Kathrin A.Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University Munich, Munich, GermanyUNSPECIFIED
Schoppmann, KathrinAnimal Ecology I and BayCEER, Bayreuth University, Bayreuth, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Fröhlich, ThomasLaboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University Munich, Munich, GermanyUNSPECIFIED
Arnold, Georg J.Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians University Munich, Munich, GermanyUNSPECIFIED
Laforsch, ChristianAnimal Ecology I and BayCEER, Bayreuth University, Bayreuth, GermanyUNSPECIFIED
Date:24 September 2015
Journal or Publication Title:npj Microgravity
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:2015
DOI :10.1038/npjmgrav.2025.16
Page Range:pp. 1-10
Publisher:Nature Publishing Group
ISSN:2373-8065
Status:Published
Keywords:Microgravity, Essential supplies, Bioregenerative life support system (BLSS), Daphina, Genomic resources, Protein
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:10 Feb 2016 09:30
Last Modified:17 Nov 2018 05:02

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