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Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat

Svejgaard, Benjamin and Wehland, Markus and Ma, Xiao and Kopp, Sascha and Sahana, Jayashree and Warnke, Elisabeth and Aleshcheva, Ganna and Hemmersbach, Ruth and Hauslage, Jens and Grosse, Jirka and Bauer, Johann and Corydon, Thomas Juhl and Islam, Tawhidul and Infanger, Manfred and Grimm, Daniela (2015) Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat. PLoS One, pp. 1-22. Public Library of Science (PLoS). DOI: 10.1371/journal.pone.0135157 ISSN 1932-6203

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4537186/

Abstract

In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)- or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1gcontrol cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin- 1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of ß1-integrin, talin-1, Ki-67, and betaactin dose-dependently in adherent cells. The ß1-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The ß-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in ß-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of ß1-integrin and talin-1 and predicts an identical effect under real microgravity conditions.

Item URL in elib:https://elib.dlr.de/102777/
Document Type:Article
Title:Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Svejgaard, BenjaminDepartment of Biomedicine, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Wehland, MarkusClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Kopp, SaschaDepartment of Biomedicine, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Sahana, JayashreeInstitute of Biomedicine, Pharmacology, Aarhus University, DK-8000 Aarhus C, DenmarkUNSPECIFIED
Warnke, ElisabethClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Aleshcheva, GannaOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Hauslage, JensGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Grosse, JirkaDepartment of Nuclear Medicine, University of Regensburg, Regensburg, GermanyUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Corydon, Thomas JuhlDepartment of Biomedicine, Aarhus University, Aarhus , DenmarkUNSPECIFIED
Islam, TawhidulDepartment of Biomedicine, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaDepartment of Biomedicine, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Date:14 August 2015
Journal or Publication Title:PLoS One
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1371/journal.pone.0135157
Page Range:pp. 1-22
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Status:Published
Keywords:Microgravity, Random Positioning Machine (RPM), 2D clinostat (CLINO), multicellular spheroids (MCS), Thyroid cancer cells
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:09 Feb 2016 12:27
Last Modified:08 Mar 2018 18:49

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