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Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity

Kopp, Sascha and Warnke, Elisabeth and Wehland, Markus and Aleshcheva, Ganna and Magnusson, Nils E. and Hemmersbach, Ruth and Corydon, Thomas Juhl and Bauer, Johann and Infanger, Manfred and Grimm, Daniela (2015) Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity. Scientific Reports (5), pp. 1-17. Nature Publishing Group. DOI: 10.1038/srep16691 ISSN 2045-2322

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649336/

Abstract

Three-dimensional multicellular spheroids (MCS) of human cells are important in cancer research. We investigated possible mechanisms of MCS formation of thyroid cells. Both, normal Nthy-ori 3–1 thyroid cells and the poorly differentiated follicular thyroid cancer cells FTC-133 formed MCS within 7 and 14 days of culturing on a Random Positioning Machine (RPM), while a part of the cells continued to grow adherently in each culture. The FTC-133 cancer cells formed larger and numerous MCS than the normal cells. In order to explain the different behaviour, we analyzed the gene expression of IL6, IL7, IL8, IL17, OPN, NGAL, VEGFA and enzymes associated cytoskeletal or membrane proteins (ACTB, TUBB, PFN1, CPNE1, TGM2, CD44, FLT1, FLK1, PKB, PKC, ERK1/2, Casp9, Col1A1) as well as the amount of secreted proteins (IL-6, IL-7, IL-8, IL-17, OPN, NGAL, VEGFA). Several of these components changed during RPM-exposure in each cell line. Striking differences between normal and malignant cells were observed in regards to the expression of genes of NGAL, VEGFA, OPN, IL6 and IL17 and to the secretion of VEGFA, IL-17, and IL-6. These results suggest several gravi-sensitive growth or angiogenesis factors being involved in 3D formation of thyroid cells cultured under simulated microgravity.

Item URL in elib:https://elib.dlr.de/102773/
Document Type:Article
Title:Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Kopp, SaschaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Warnke, ElisabethClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Wehland, MarkusClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Aleshcheva, GannaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Magnusson, Nils E.Medical Research Laboratory, Department of Clinical Medicine, Aarhus University, 8000 Aarhus C, DenmarkUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Corydon, Thomas JuhlDepartment of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, Aarhus C, DK-8000, DenmarkUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaDepartment of Biomedicine, Aarhus University, DK 8000 Aarhus C, DenmarkUNSPECIFIED
Date:18 November 2015
Journal or Publication Title:Scientific Reports
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1038/srep16691
Page Range:pp. 1-17
Publisher:Nature Publishing Group
ISSN:2045-2322
Status:Published
Keywords:Microgravity, Thyroid cells, Health problems, 3D growth, Thyroid cancer cells, Random Positioning Machine (RPM)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:03 Feb 2016 10:15
Last Modified:08 Mar 2018 18:36

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