elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

2-D Clinostat for Simulated Microgravity Experiments with Arabidopsis Seedlings

Wang, Hui and Li, Xugang and Krause, Lars and Görög, Mark and Schüler, Oliver and Hauslage, Jens and Hemmersbach, Ruth and Kirchner, Stefan and Lasok, Hanna and Haser, Thomas and Rapp, Katja and Schmidt, Jürgen and Yu, Xin and Pasternak, Taras and Aubry-Hivet, Dorothee and Tietz, Olaf and Dovzhenko, Alexander and Palme, Klaus and Anicet Ditengou, Franck (2015) 2-D Clinostat for Simulated Microgravity Experiments with Arabidopsis Seedlings. Microgravity Science and Technology, pp. 1-8. Springer. DOI: 10.1007/s12217-015-9478-1 ISSN 0938-0108

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1007/s12217-015-9478-1

Abstract

Ground-based simulators of microgravity such as fast rotating 2-D clinostats are valuable tools to study gravity related processes. We describe here a versatile g-value-adjustable 2-D clinostat that is suitable for plant analysis. To avoid seedling adaptation to 1 g after clinorotation, we designed chambers that allow rapid fixation. A detailed protocol for fixation, RNA isolation and the analysis of selected genes is described. Using this clinostat we show that mRNA levels of LONG HYPOCOTYL 5 (HY5), MIZU-KUSSEI 1 (MIZ1) and microRNA MIR163 are down- regulated in 5-day-old Arabidopsis thaliana roots after 3 min and 6 min of clinorotation using a maximal reduced g-force of 0.02 g, hence demonstrating that this 2-D clinostat enables the characterization of early transcriptomic events during root response to microgravity. We further show that this 2-D clinostat is able to compensate the action of gravitational force as both gravitropic-dependent statolith sedimentation and subsequent auxin redistribution (monitoring DR5rev :: GFP reporter) are abolished when plants are clinorotated. Our results demonstrate that 2-D clinostats equipped with interchangeable growth chambers and tunable rotation velocity are suitable for studying how plants perceive and respond to simulated microgravity.

Item URL in elib:https://elib.dlr.de/102761/
Document Type:Article
Title:2-D Clinostat for Simulated Microgravity Experiments with Arabidopsis Seedlings
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Wang, HuiInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Li, XugangInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Krause, LarsGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, GermanyUNSPECIFIED
Görög, MarkGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Schüler, OliverGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Kirchner, StefanInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Lasok, HannaInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Haser, ThomasInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Rapp, KatjaInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Schmidt, JürgenInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Yu, XinInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Pasternak, TarasInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Aubry-Hivet, DorotheeInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, GermanyUNSPECIFIED
Tietz, OlafInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Dovzhenko, AlexanderInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Palme, KlausInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, GermanyUNSPECIFIED
Anicet Ditengou, FranckInstitute for Biology II, Molecular Plant Physiology, Faculty of Biology, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, D-79104 Freiburg, GermanyUNSPECIFIED
Date:9 June 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9478-1
Page Range:pp. 1-8
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Simulated Microgravity, Arabidopsis thaliana, Clinostat, Gene expression, Gravity, Auxin, Signal transduction
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:03 Feb 2016 10:11
Last Modified:10 May 2016 23:44

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.