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Proper selection of 1g controls in simulated microgravity research as illustrated with clinorotated plant cell suspension cultures

Kamal, Khaled Y and Hemmersbach, Ruth and Medina, Francisco Javier and Herranz, Raul (2015) Proper selection of 1g controls in simulated microgravity research as illustrated with clinorotated plant cell suspension cultures. Life Sciences in Space Research, pp. 47-52. Elsevier. DOI: 10.1016/j.lssr.2015.04.004 ISSN 2214-5524

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Official URL: http://dx.doi.org/10.1016/j.lssr.2015.04.004

Abstract

Understanding the physical and biological effects of the absence of gravity is necessary to conduct operations on space environments. It has been previously shown that the microgravity environment induces the dissociation of cell proliferation from cell growth in young seedling root meristems, but this source material is limited to few cells in each row of meristematic layers. Plant cell cultures, composed by a large and homogeneous population of proliferating cells, are an ideal model to study the effects of altered gravity on cellular mechanisms regulating cell proliferation and associated cell growth. Cell suspension cultures of Arabidopsis thaliana cell line (MM2d) were exposed to 2D-clinorotation in a pipette clinostat for 3.5 or 14 h, respectively, and were then processed either by quick freezing, to be used in flow cytometry, or by chemical fixation, for microscopy techniques. After long-term clinorotation, the proportion of cells in G1 phase was increased and the nucleolus area, as revealed by immunofluorescence staining with anti-nucleolin, was decreased. Despite the compatibility of these results with those obtained in real microgravity on seedling meristems, we provide a technical discussion in the context of clinorotation and proper 1 g controls with respect to suspension cultures. Standard 1 g procedure of sustaining the cell suspension is achieved by continuously shaking. Thus, we compare the mechanical forces acting on cells in clinorotated samples, in a control static sample and in the standard 1 g conditions of suspension cultures in order to define the conditions of a complete and reliable experiment in simulated microgravity with corresponding 1 g controls.

Item URL in elib:https://elib.dlr.de/102760/
Document Type:Article
Title:Proper selection of 1g controls in simulated microgravity research as illustrated with clinorotated plant cell suspension cultures
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Kamal, Khaled YCentro de Investigaciones Biológicas (CSIC), Madrid, SpainUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Medina, Francisco JavierConsejo Superior de Investigaciones Cientificas (CSIC), Centro de Investigaciones Biológicas, Madrid, SpainUNSPECIFIED
Herranz, RaulCentro de Investigaciones Biológicas (CSIC), Madrid, Spain; rherranz (at) csic.esUNSPECIFIED
Date:24 March 2015
Journal or Publication Title:Life Sciences in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:No
DOI :10.1016/j.lssr.2015.04.004
Page Range:pp. 47-52
Publisher:Elsevier
ISSN:2214-5524
Status:Published
Keywords:Simulated microgravity, Ground-based facilities, 2D clinostat, Shear forces, Arabidopsis thaliana, Suspension cell culture
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:03 Feb 2016 10:04
Last Modified:10 May 2016 23:44

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