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Changes in Gene Expression of Arabidopsis Thaliana Cell Cultures Upon Exposure to Real and Simulated Partial-g Forces

Fengler, Svenja and Spirer, Ina and Neef, Maren and Margret, Ecke and Hauslage, Jens and Hampp, Rüdiger (2015) Changes in Gene Expression of Arabidopsis Thaliana Cell Cultures Upon Exposure to Real and Simulated Partial-g Forces. Microgravity Science and Technology, pp. 1-11. Springer. DOI: 10.1007/s12217-015-9452-y ISSN 0938-0108

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1007/s12217-015-9452-y

Abstract

Cell cultures of the plant model organism Arabidopsis thaliana were exposed to partial-g forces during parabolic flight and clinostat experiments (0.16 g, 0.38 g and 0.5 g were tested). In order to investigate gravity-dependent alterations in gene expression, samples were metabolically quenched by the fixative RNAlater to stabilize nucleic acids and used for whole-genome microarray analysis. An attempt to identify the potential threshold acceleration for the gravity-dependent response showed that the smaller the experienced g-force, the greater was the susceptibility of the cell cultures. Compared to short-term μg during a parabolic flight, the number of differentially expressed genes under partial-g was lower. In addition, the effect on the alteration of amounts of transcripts decreased during partial-g parabolic flight due to the sequence of the different parabolas (0.38 g, 0.16 g and μg). A time-dependent analysis under simulated 0.5 g indicates that adaptation occurs within minutes. Differentially expressed genes (at least 2-fold up- or down-regulated in expression) under real flight conditions were to some extent identical with those affected by clinorotation. The highest number of homologuous genes was detected within seconds of exposure to 0.38 g (both flight and clinorotation). To a considerable part, these genes deal with cell wall properties. Additionally, responses specific for clinorotation were observed.

Item URL in elib:https://elib.dlr.de/102759/
Document Type:Article
Title:Changes in Gene Expression of Arabidopsis Thaliana Cell Cultures Upon Exposure to Real and Simulated Partial-g Forces
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Fengler, SvenjaUniversity of Tübingen, Physiological Ecology of Plants, Tübingen, GermanyUNSPECIFIED
Spirer, InaUniversity of Tübingen, Physiological Ecology of Plants, Tübingen, GermanyUNSPECIFIED
Neef, MarenUniversity of Tübingen, Physiological Ecology of Plants, Tübingen, GermanyUNSPECIFIED
Margret, EckeUniversity of Tübingen, Physiological Ecology of Plants, Tübingen, GermanyUNSPECIFIED
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Hampp, RüdigerUniversity of Tübingen, Physiological Ecology of Plants, Tübingen, GermanyUNSPECIFIED
Date:5 September 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9452-y
Page Range:pp. 1-11
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Microgravity, Arabidopsis thaliana, Cell cultures, Clinostat, Gene expression, Parabolic flight, Partial-g forces
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:03 Feb 2016 10:01
Last Modified:10 May 2016 23:44

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