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Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes

Unruh, Eckehardt und Brungs, Sonja und Langer, Samantha und Bornemann, Gerhild und Frett, Timo und Hansen, Peter-Diedrich (2015) Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes. Microgravity Science and Technology. Springer. doi: 10.1007/s12217-015-9438-9. ISSN 0938-0108.

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Offizielle URL: http://dx.doi.org/10.1007/s12217-015-9438-9

Kurzfassung

Microgravity induces alterations in the functioning of immune cell; however, the underlying mechanisms have not yet been identified. In this study, hemocytes (blood cells) of the blue mussel Mytilus edulis were investigated under altered gravity conditions. The study was conducted on the ground in preparation for the BIOLAB TripleLux-B experiment, which will be performed on the International Space Station (ISS). On-line kinetic measurements of reactive oxygen species (ROS) production during the oxidative burst and thus cellular activity of isolated hemocytes were performed in a photomultiplier (PMT)-clinostat (simulated microgravity) and in the 1g operation mode of the clinostat in hypergravity on the Short-Arm Human Centrifuge (SAHC) as well as during parabolic flights. In addition to studies with isolated hemocytes, the effect of altered gravity conditions on whole animals was investigated. For this purpose, whole mussels were exposed to hypergravity (1.8 g) on a multi-sample incubator centrifuge (MuSIC) or to simulated microgravity in a submersed clinostat. After exposure for 48 h, hemocytes were taken from the mussels and ROS production was measured under 1 g conditions. The results from the parabolic flights and clinostat studies indicate that mussel hemocytes respond to altered gravity in a fast and reversible manner. Hemocytes (after cryo-conservation) exposed to simulated microgravity (μg), as well as fresh hemocytes from clinorotated animals, showed a decrease in ROS production. Measurements during a permanent exposure of hemocytes to hypergravity (SAHC) show a decrease in ROS production. Hemocytes of mussels measured after the centrifugation of whole mussels did not show an influence to the ROS response at all. Hypergravity during parabolic flights led to a decrease but also to an increase in ROS production in isolated hemocytes, whereas the centrifugation of whole mussels did not influence the ROS response at all. This study is a good example how ground-based facility experiments can be used to prepare for an upcoming ISS experiment, in this case the TRIPLE LUX B experiment.

elib-URL des Eintrags:https://elib.dlr.de/102757/
Dokumentart:Zeitschriftenbeitrag
Titel:Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Unruh, EckehardtBerlin Institute of Technology (BIT), Department of Ecological Impact Research and Ecotoxicology, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Brungs, SonjaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Langer, SamanthaBerlin Institute of Technology (BIT), Department of Ecological Impact Research and Ecotoxicology, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Bornemann, GerhildDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Frett, TimoGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Hansen, Peter-DiedrichTechnische Universität Berlin (BIT - Berlin Institute of Technology) Department of Ecotoxicology, Franklinstraße 29 (OE4), 10587, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Datum:21 Juni 2015
Erschienen in:Microgravity Science and Technology
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
DOI:10.1007/s12217-015-9438-9
Verlag:Springer
ISSN:0938-0108
Status:veröffentlicht
Stichwörter:Phagocytosis, Reactive oxygen species (ROS), Microgravity, Hypergravity, Parabolic flight, Clinostat
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Biomedizinische Forschung
Hinterlegt von: Hoven, Claudio
Hinterlegt am:03 Feb 2016 09:53
Letzte Änderung:10 Mai 2016 23:44

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