elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes

Unruh, Eckehardt and Brungs, Sonja and Langer, Samantha and Bornemann, Gerhild and Frett, Timo and Hansen, Peter-Diedrich (2015) Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes. Microgravity Science and Technology. Springer. DOI: 10.1007/s12217-015-9438-9 ISSN 0938-0108

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1007/s12217-015-9438-9

Abstract

Microgravity induces alterations in the functioning of immune cell; however, the underlying mechanisms have not yet been identified. In this study, hemocytes (blood cells) of the blue mussel Mytilus edulis were investigated under altered gravity conditions. The study was conducted on the ground in preparation for the BIOLAB TripleLux-B experiment, which will be performed on the International Space Station (ISS). On-line kinetic measurements of reactive oxygen species (ROS) production during the oxidative burst and thus cellular activity of isolated hemocytes were performed in a photomultiplier (PMT)-clinostat (simulated microgravity) and in the 1g operation mode of the clinostat in hypergravity on the Short-Arm Human Centrifuge (SAHC) as well as during parabolic flights. In addition to studies with isolated hemocytes, the effect of altered gravity conditions on whole animals was investigated. For this purpose, whole mussels were exposed to hypergravity (1.8 g) on a multi-sample incubator centrifuge (MuSIC) or to simulated microgravity in a submersed clinostat. After exposure for 48 h, hemocytes were taken from the mussels and ROS production was measured under 1 g conditions. The results from the parabolic flights and clinostat studies indicate that mussel hemocytes respond to altered gravity in a fast and reversible manner. Hemocytes (after cryo-conservation) exposed to simulated microgravity (μg), as well as fresh hemocytes from clinorotated animals, showed a decrease in ROS production. Measurements during a permanent exposure of hemocytes to hypergravity (SAHC) show a decrease in ROS production. Hemocytes of mussels measured after the centrifugation of whole mussels did not show an influence to the ROS response at all. Hypergravity during parabolic flights led to a decrease but also to an increase in ROS production in isolated hemocytes, whereas the centrifugation of whole mussels did not influence the ROS response at all. This study is a good example how ground-based facility experiments can be used to prepare for an upcoming ISS experiment, in this case the TRIPLE LUX B experiment.

Item URL in elib:https://elib.dlr.de/102757/
Document Type:Article
Title:Comprehensive Study of the Influence of Altered Gravity on the Oxidative Burst of Mussel (Mytilus edulis) Hemocytes
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Unruh, EckehardtBerlin Institute of Technology (BIT), Department of Ecological Impact Research and Ecotoxicology, Berlin, GermanyUNSPECIFIED
Brungs, SonjaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyUNSPECIFIED
Langer, SamanthaBerlin Institute of Technology (BIT), Department of Ecological Impact Research and Ecotoxicology, Berlin, GermanyUNSPECIFIED
Bornemann, GerhildDLR Institute of Aerospace Medicine, Biomedical Research, Cologne, GermanyUNSPECIFIED
Frett, TimoGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, GermanyUNSPECIFIED
Hansen, Peter-DiedrichTechnische Universität Berlin (BIT - Berlin Institute of Technology) Department of Ecotoxicology, Franklinstraße 29 (OE4), 10587, Berlin, GermanyUNSPECIFIED
Date:21 June 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9438-9
Publisher:Springer
ISSN:0938-0108
Status:Published
Keywords:Phagocytosis, Reactive oxygen species (ROS), Microgravity, Hypergravity, Parabolic flight, Clinostat
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Hoven, Claudio
Deposited On:03 Feb 2016 09:53
Last Modified:10 May 2016 23:44

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.