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Alterations of the cytoskeleton in human cells in space proved by life-cell imaging

Corydon, Thoma J. and Kopp, Sascha and Wehland, Markus and Braun, Markus and Schütte, Andrea and Meyer, Tobias and Hülsing, Thomas and Oltmann, Hergen and Schmitz, Burkhard and Hemmersbach, Ruth and Grimm, Daniela (2016) Alterations of the cytoskeleton in human cells in space proved by life-cell imaging. Scientific Reports, pp. 1-14. Nature Publishing Group. DOI: 10.1038/srep20043 ISSN 2045-2322

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Official URL: http://www.nature.com/articles/srep20043

Abstract

Microgravity induces changes in the cytoskeleton. This might have an impact on cells and organs of humans in space. Unfortunately, studies of cytoskeletal changes in microgravity reported so far are obligatorily based on the analysis of fixed cells exposed to microgravity during a parabolic flight campaign (PFC). This study focuses on the development of a compact fluorescence microscope (FLUMIAS) for fast live-cell imaging under real microgravity. It demonstrates the application of the instrument for on-board analysis of cytoskeletal changes in FTC-133 cancer cells expressing the Lifeact-GFP marker protein for the visualization of F-actin during the 24th DLR PFC and TEXUS 52 rocket mission. Although vibration is an inevitable part of parabolic flight maneuvers, we successfully for the first time report life-cell cytoskeleton imaging during microgravity, and gene expression analysis after the 31st parabola showing a clear up-regulation of cytoskeletal genes. Notably, during the rocket flight the FLUMIAS microscope reveals significant alterations of the cytoskeleton related to microgravity. Our findings clearly demonstrate the applicability of the FLUMIAS microscope for life-cell imaging during microgravity, rendering it an important technological advance in live-cell imaging when dissecting protein localization.

Item URL in elib:https://elib.dlr.de/102497/
Document Type:Article
Title:Alterations of the cytoskeleton in human cells in space proved by life-cell imaging
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Corydon, Thoma J.Aarhus University, Department of Biomedicine, Aarhus, DenmarkUNSPECIFIED
Kopp, SaschaUniversity of Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Wehland, MarkusUniversity of Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Braun, MarkusUniversity of Bonn, Institute for Molecular Physiology and Biotechnology of Plants (IMBIO), Gravitational Biology Group, Bonn, GermanyUNSPECIFIED
Schütte, AndreaAirbus Defense and Space, Airbus DS GmbH, Bremen, GermanyUNSPECIFIED
Meyer, TobiasFEI Munich GmbH, Gräfeling, GermanyUNSPECIFIED
Hülsing, ThomasAirbus Defense and Space, Airbus DS GmbH, Bremen, GermanyUNSPECIFIED
Oltmann, HergenAirbus Defense and Space, Airbus DS GmbH, Bremen, GermanyUNSPECIFIED
Schmitz, BurkhardAirbus Defense and Space, Airbus DS GmbH, Bremen, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Grimm, DanielaAarhus University, Department of Biomedicine, Aarhus, DenmarkUNSPECIFIED
Date:24 February 2016
Journal or Publication Title:Scientific Reports
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI :10.1038/srep20043
Page Range:pp. 1-14
Publisher:Nature Publishing Group
ISSN:2045-2322
Status:Published
Keywords:Cytoskeleton, Microgravity, Live-cell imaging, FLUMIAS microscope, F-actin
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:15 Mar 2016 11:23
Last Modified:15 Mar 2016 11:23

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