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Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station

Onofri, Silvano and de Vera, Jean Pierre Paul and Zucconi, Laura and Selbmann, Laura and Scalzi, Giuliano and Venkateswaran, Kasthuri J. and Rabbow, Elke and de la Torre, Rosa and Horneck, Gerda (2015) Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station. Astrobiology, 15 (12), pp. 1052-1059. Mary Ann Liebert Inc.. DOI: 10.1089/ast.2015.1324 ISSN 1531-1074

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1089/ast.2015.1324

Abstract

Dehydrated Antarctic cryptoendolithic communities and colonies of the rock inhabitant black fungi Cryomyces antarcticus (CCFEE 515) and Cryomyces minteri (CCFEE 5187) were exposed as part of the Lichens and Fungi Experiment (LIFE) for 18 months in the European Space Agency's EXPOSE-E facility to simulated martian conditions aboard the International Space Station (ISS). Upon sample retrieval, survival was proved by testing colony-forming ability, and viability of cells (as integrity of cell membrane) was determined by the propidium monoazide (PMA) assay coupled with quantitative PCR tests. Although less than 10% of the samples exposed to simulated martian conditions were able to proliferate and form colonies, the PMA assay indicated that more than 60% of the cells and rock communities had remained intact after the “Mars exposure.” Furthermore, a high stability of the DNA in the cells was demonstrated. The results contribute to assessing the stability of resistant microorganisms and biosignatures on the surface of Mars, data that are valuable information for further search-for-life experiments on Mars.

Item URL in elib:https://elib.dlr.de/102184/
Document Type:Article
Title:Survival of Antarctic Cryptoendolithic Fungi in Simulated Martian Conditions On Board the International Space Station
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Onofri, SilvanoDepartment of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.; onofri (at) unitus.itUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Zucconi, LauraDepartment of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.UNSPECIFIED
Selbmann, LauraDepartment of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.UNSPECIFIED
Scalzi, GiulianoDepartment of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy.UNSPECIFIED
Venkateswaran, Kasthuri J.Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA.UNSPECIFIED
Rabbow, ElkeInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
de la Torre, RosaDepartment of Earth Observation, Spanish Aerospace Research Establishment-INTA, Torrejón de Ardoz, Madrid, Spain.UNSPECIFIED
Horneck, GerdaInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Date:2015
Journal or Publication Title:Astrobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:15
DOI :10.1089/ast.2015.1324
Page Range:pp. 1052-1059
Publisher:Mary Ann Liebert Inc.
ISSN:1531-1074
Status:Published
Keywords:Endoliths, Eukaryotes, Extremophilic microorganisms, Mars, Radiation resistance
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Exploration des Sonnensystems, R - Vorhaben Strahlenbiologie, R - Projekt ISS Nutzung in der Biodiagnostik
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:14 Jan 2016 15:34
Last Modified:06 Sep 2019 15:23

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