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Tissue Engineering of Cartilage on Ground-Based Facilities

Aleshcheva, Ganna and Bauer, Johann and Hemmersbach, Ruth and Egli, Marcel and Wehland, Markus and Grimm, Daniela (2015) Tissue Engineering of Cartilage on Ground-Based Facilities. Microgravity Science and Technology. Springer. DOI: 10.1007/s12217-015-9479-0 ISSN 0938-0108

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Official URL: http://dx.doi.org/10.1007/s12217-015-9479-0


Investigations under simulated microgravity offer the opportunity for a better understanding of the influence of altered gravity on cells and the scaffold-free three- dimensional (3D) tissue formation. To investigate the short- term influence, human chondrocytes were cultivated for 2 h, 4 h, 16 h, and 24 h on a 2D Fast-Rotating Clinostat (FRC) in DMEM/F-12 medium supplemented with 10 % FCS. We detected holes in the vimentin network, perinuclear accumulations of vimentin after 2 h, and changes in the chondrocytes shape visualised by F-actin staining after 4 h of FRC-exposure. Scaffold-free cultivation of chondrocytes for 7 d on the Random Positioning Machine (RPM), the FRC and the Rotating Wall Vessel (RWV) resulted in spheroid formation, a phenomenon already known from spaceflight experiments with chondrocytes (MIR Space Station) and thyroid cancer cells (SimBox/Shenzhou-8 space mission). The experiments enabled by the ESA-CORA- GBF programme gave us an optimal opportunity to study gravity-related cellular processes, validate ground-based facilities for our chosen cell system, and prepare long-term experiments under real microgravity conditions in space.

Item URL in elib:https://elib.dlr.de/101720/
Document Type:Article
Title:Tissue Engineering of Cartilage on Ground-Based Facilities
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Aleshcheva, GannaSpacelife, Otto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Bauer, JohannMax-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Egli, MarcelAerospace Biomedical Science & Technology, Space Biology Group, Lucerne University of Applied Sciences and Arts, SwitzerlandUNSPECIFIED
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, Magdeburg, GermanyUNSPECIFIED
Date:29 December 2015
Journal or Publication Title:Microgravity Science and Technology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In ISI Web of Science:Yes
DOI :10.1007/s12217-015-9479-0
Keywords:Microgravity, Random Positioning Machine, Clinostat, Rotating Wall Vessel, Chondrocytes, Cartilage, Cytoskeleton, Tissue engineering
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Research
Deposited By: Duwe, Helmut
Deposited On:03 Feb 2016 09:43
Last Modified:10 May 2016 23:40

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