Aleshcheva, Ganna und Bauer, Johann und Hemmersbach, Ruth und Egli, Marcel und Wehland, Markus und Grimm, Daniela (2015) Tissue Engineering of Cartilage on Ground-Based Facilities. Microgravity Science and Technology. Springer. doi: 10.1007/s12217-015-9479-0. ISSN 0938-0108.
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Offizielle URL: http://dx.doi.org/10.1007/s12217-015-9479-0
Kurzfassung
Investigations under simulated microgravity offer the opportunity for a better understanding of the influence of altered gravity on cells and the scaffold-free three- dimensional (3D) tissue formation. To investigate the short- term influence, human chondrocytes were cultivated for 2 h, 4 h, 16 h, and 24 h on a 2D Fast-Rotating Clinostat (FRC) in DMEM/F-12 medium supplemented with 10 % FCS. We detected holes in the vimentin network, perinuclear accumulations of vimentin after 2 h, and changes in the chondrocytes shape visualised by F-actin staining after 4 h of FRC-exposure. Scaffold-free cultivation of chondrocytes for 7 d on the Random Positioning Machine (RPM), the FRC and the Rotating Wall Vessel (RWV) resulted in spheroid formation, a phenomenon already known from spaceflight experiments with chondrocytes (MIR Space Station) and thyroid cancer cells (SimBox/Shenzhou-8 space mission). The experiments enabled by the ESA-CORA- GBF programme gave us an optimal opportunity to study gravity-related cellular processes, validate ground-based facilities for our chosen cell system, and prepare long-term experiments under real microgravity conditions in space.
elib-URL des Eintrags: | https://elib.dlr.de/101720/ | ||||||||||||||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||||||||||
Titel: | Tissue Engineering of Cartilage on Ground-Based Facilities | ||||||||||||||||||||||||||||
Autoren: |
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Datum: | 29 Dezember 2015 | ||||||||||||||||||||||||||||
Erschienen in: | Microgravity Science and Technology | ||||||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||||||
Open Access: | Nein | ||||||||||||||||||||||||||||
Gold Open Access: | Nein | ||||||||||||||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||||||||||||||
DOI: | 10.1007/s12217-015-9479-0 | ||||||||||||||||||||||||||||
Verlag: | Springer | ||||||||||||||||||||||||||||
ISSN: | 0938-0108 | ||||||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||||||
Stichwörter: | Microgravity, Random Positioning Machine, Clinostat, Rotating Wall Vessel, Chondrocytes, Cartilage, Cytoskeleton, Tissue engineering | ||||||||||||||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt) | ||||||||||||||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Biomedizinische Forschung | ||||||||||||||||||||||||||||
Hinterlegt von: | Duwe, Helmut | ||||||||||||||||||||||||||||
Hinterlegt am: | 03 Feb 2016 09:43 | ||||||||||||||||||||||||||||
Letzte Änderung: | 10 Mai 2016 23:40 |
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