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Collagen type III and VI turnover in response to long-term immobilization

Sun, Shu and Henriksen, K. and Karsdal, M. A. and Byrjalsen, Inger and Rittweger, Jörn and Armbrecht, Gabriele and Belavy, Daniel and Felsenberg, Dieter and Nedergaard, A. F. (2015) Collagen type III and VI turnover in response to long-term immobilization. PLoS One, 10 (2). Public Library of Science (PLoS). DOI: 10.1371/journal.pone.0144525 ISSN 1932-6203

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Abstract

BACKGROUND: Muscle mass and function are perturbed by immobilization and remobilization. When muscle mass changes, the quality and quantity of the extracellular matrix protein, particularly the collagens, change with it. In this study, we investigated the temporal profile of three peptide biomarkers derived from turnover of collagen type III and type VI in a long-term immobilization and remobilization study. We also compared individual biomarker levels with Lean body Mass (LBM) and changes therein, hypothesizing that these biomarkers would be biomarkers of the remodeling processes associated with immobilization and/or remobilization. METHODS: In the Berlin bed rest study, 20 young men were recruited and randomly assigned to 8-week's strict bed rest with or without resistive vibration exercise countermeasure. We measured three neo-epitope ELISA kits in the serum samples of this study: Pro-C3, measured the synthesis of collagen type III; Pro-C6, measured the synthesis of collagen type VI; and C6M measured the degradation of collagen type VI induced by MMP-2 and MMP-9 cleavage. RESULTS: Pro-C3 and Pro-C6 biomarkers are up-regulated with both immobilization and remobilization, whereas C6M is hardly affected at all. We found that Pro-C3 and C6M levels are related to LBM at baseline and that high levels of Pro-C6 are associated with smaller changes in muscle mass during both immobilization and remobilization. CONCLUSION: The Pro-C3 and-C6 biomarkers change likely reflect remodeling changes in response to unloading or reloading, whereas C6M does not appear to respond to unloading. Pro-C3 and C6M levels correlate with LBM at baseline, while Pro-C6 is related to the anabolic and catabolic responses to unloading and reloading.

Item URL in elib:https://elib.dlr.de/100989/
Document Type:Article
Title:Collagen type III and VI turnover in response to long-term immobilization
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Sun, ShuNordic Bioscience Biomarkers and Research, Herlev, DenmarkUNSPECIFIED
Henriksen, K.Nordic Bioscience Biomarkers and Research, Herlev, DenmarkUNSPECIFIED
Karsdal, M. A.Nordic Bioscience Biomarkers and Research, Herlev, DenmarkUNSPECIFIED
Byrjalsen, IngerNordic Bioscience Biomarkers and Research, Herlev, DenmarkUNSPECIFIED
Rittweger, JörnInstitute of Aerospace Medicine, Deutsches Zentrum für Luft- und Raumfahrt, DLR e.V., Cologne, Germany and Medical Faculty, University of Cologne, Cologne, Germany; joern.rittweger (at) dlr.deUNSPECIFIED
Armbrecht, GabrieleCharité Campus Benjamin FranklinUNSPECIFIED
Belavy, DanielCharité University Medicine BerlinUNSPECIFIED
Felsenberg, DieterCharité Campus Benjamin FranklinUNSPECIFIED
Nedergaard, A. F.Nordic Bioscience Biomarkers and Research, Herlev, DenmarkUNSPECIFIED
Date:7 December 2015
Journal or Publication Title:PLoS One
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI :10.1371/journal.pone.0144525
Editors:
EditorsEmail
Gulberg, DonaldUniversity of Bergen, NORWAY
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Status:Published
Keywords:Long-Term Immobilization; remobilization; Collagen; extracellular matrix protein
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Becker, Christine
Deposited On:07 Jan 2016 16:48
Last Modified:08 Mar 2018 18:49

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