Cortés-Sánchez, J.L. und Melnik, D. und Sandt, V. und Kahlert, S. und Marchal, S. und Johnson, I. und Calvaruso, M. und Liemersdorf, C. und Wuest, S. und Grimm, D. und Krüger, M. (2023) Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine. Cells, 12 (22), Seite 2665. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/cells12222665. ISSN 2073-4409.
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Offizielle URL: https://doi.org/10.3390/cells12222665
Kurzfassung
In preparing space and microgravity experiments, the utilization of ground-based facilities is common for initial experiments and feasibility studies. One approach to simulating microgravity conditions on Earth is to employ a random positioning machine (RPM) as a rotary bioreactor. Combined with a suitable low-mass model system, such as cell cultures, these devices simulating microgravity have been shown to produce results similar to those obtained in a space experiment under real microgravity conditions. One of these effects observed under real and simulated microgravity is the formation of spheroids from 2D adherent cancer cell cultures. Since real microgravity cannot be generated in a laboratory on Earth, we aimed to determine which forces lead to the detachment of individual FTC-133 thyroid cancer cells and the formation of tumor spheroids during culture with exposure to random positioning modes. To this end, we subdivided the RPM motion into different static and dynamic orientations of cell culture flasks. We focused on the molecular activation of the mechanosignaling pathways previously associated with spheroid formation in microgravity. Our results suggest that RPM-induced spheroid formation is a two-step process. First, the cells need to be detached, induced by the cell culture flask’s rotation and the subsequent fluid flow, as well as the presence of air bubbles. Once the cells are detached and in suspension, random positioning prevents sedimentation, allowing 3D aggregates to form. In a comparative shear stress experiment using defined fluid flow paradigms, transcriptional responses were triggered comparable to exposure of FTC-133 cells to the RPM. In summary, the RPM serves as a simulator of microgravity by randomizing the impact of Earth’s gravity vector especially for suspension (i.e., detached) cells. Simultaneously, it simulates physiological shear forces on the adherent cell layer. The RPM thus offers a unique combination of environmental conditions for in vitro cancer research.
elib-URL des Eintrags: | https://elib.dlr.de/202872/ | ||||||||||||||||||||||||||||||||||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||||||||||||||||||||||||||||||
Titel: | Fluid and Bubble Flow Detach Adherent Cancer Cells to Form Spheroids on a Random Positioning Machine | ||||||||||||||||||||||||||||||||||||||||||||||||
Autoren: |
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Datum: | 20 November 2023 | ||||||||||||||||||||||||||||||||||||||||||||||||
Erschienen in: | Cells | ||||||||||||||||||||||||||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||||||||||||||||||||||||||
Open Access: | Ja | ||||||||||||||||||||||||||||||||||||||||||||||||
Gold Open Access: | Ja | ||||||||||||||||||||||||||||||||||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||||||||||||||||||||||||||||||||||
Band: | 12 | ||||||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.3390/cells12222665 | ||||||||||||||||||||||||||||||||||||||||||||||||
Seitenbereich: | Seite 2665 | ||||||||||||||||||||||||||||||||||||||||||||||||
Herausgeber: |
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Verlag: | Multidisciplinary Digital Publishing Institute (MDPI) | ||||||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 2073-4409 | ||||||||||||||||||||||||||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||||||||||||||||||||||||||
Stichwörter: | rotating bioreactor; simulated microgravity; cancer cell; shear stress; cell detachment; in vitro metastasis | ||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr | ||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Programm: | Raumfahrt | ||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Programmthema: | Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Schwerpunkt: | Raumfahrt | ||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | R - Gravisensorik | ||||||||||||||||||||||||||||||||||||||||||||||||
Standort: | Köln-Porz | ||||||||||||||||||||||||||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie | ||||||||||||||||||||||||||||||||||||||||||||||||
Hinterlegt von: | Anken, Ralf | ||||||||||||||||||||||||||||||||||||||||||||||||
Hinterlegt am: | 20 Feb 2024 07:58 | ||||||||||||||||||||||||||||||||||||||||||||||||
Letzte Änderung: | 26 Feb 2024 11:48 |
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