Hövels, Marcel und Gallala, Nicole und Keriakes, Samara Lisa und König, Anna Paulina und Schiessl, Jacqueline und Laporte, Tobias und Kosciow, Konrad und Deppenmeier, Uwe (2022) 5-Keto-D-Fructose, a Natural Diketone and Potential Sugar Substitute, Significantly Reduces the Viability of Prokaryotic and Eukaryotic Cells. Frontiers in Microbiology, 13 (935062). Frontiers Media S.A.. doi: 10.3389/fmicb.2022.935062. ISSN 1664-302X.
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Offizielle URL: https://www.frontiersin.org/articles/10.3389/fmicb.2022.935062/full
Kurzfassung
5-Keto-D-fructose (5-KF) is a natural diketone occurring in micromolar concentrations in honey, white wine, and vinegar. The oxidation of D-fructose to 5-KF is catalyzed by the membrane-bound fructose dehydrogenase complex found in several acetic acid bacteria. Since 5-KF has a sweetening power comparable to fructose and is presumably calorie-free, there is great interest in making the diketone commercially available as a new sugar substitute. Based on a genetically modified variant of the acetic acid bacterium Gluconobacter oxydans 621H, an efficient process for the microbial production of 5-KF was recently developed. However, data on the toxicology of the compound are completely lacking to date. Therefore, this study aimed to investigate the effect of 5-KF on the viability of prokaryotic and eukaryotic cells. It was found that the compound significantly inhibited the growth of the gram-positive and gram-negative model organisms Bacillus subtilis and Escherichia coli in a concentration-dependent manner. Furthermore, cell viability assays confirmed severe cytotoxicity of 5-KF toward the colon cancer cell line HT-29. Since these effects already occurred at concentrations of 5 mM, the use of 5-KF in the food sector should be avoided. The studies performed revealed that in the presence of amines, 5-KF promoted a strong Maillard reaction. The inherent reactivity of 5-KF as well as the Maillard products formed could be the trigger for the observed inhibition of prokaryotic and eukaryotic cells.
elib-URL des Eintrags: | https://elib.dlr.de/194789/ | ||||||||||||||||||||||||||||||||||||
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Dokumentart: | Zeitschriftenbeitrag | ||||||||||||||||||||||||||||||||||||
Titel: | 5-Keto-D-Fructose, a Natural Diketone and Potential Sugar Substitute, Significantly Reduces the Viability of Prokaryotic and Eukaryotic Cells | ||||||||||||||||||||||||||||||||||||
Autoren: |
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Datum: | 21 Juni 2022 | ||||||||||||||||||||||||||||||||||||
Erschienen in: | Frontiers in Microbiology | ||||||||||||||||||||||||||||||||||||
Referierte Publikation: | Ja | ||||||||||||||||||||||||||||||||||||
Open Access: | Ja | ||||||||||||||||||||||||||||||||||||
Gold Open Access: | Ja | ||||||||||||||||||||||||||||||||||||
In SCOPUS: | Ja | ||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | Ja | ||||||||||||||||||||||||||||||||||||
Band: | 13 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.3389/fmicb.2022.935062 | ||||||||||||||||||||||||||||||||||||
Verlag: | Frontiers Media S.A. | ||||||||||||||||||||||||||||||||||||
ISSN: | 1664-302X | ||||||||||||||||||||||||||||||||||||
Status: | veröffentlicht | ||||||||||||||||||||||||||||||||||||
Stichwörter: | 5-keto-D-fructose, toxicology, Maillard reaction, sugar substitutes, HT-29 cell line | ||||||||||||||||||||||||||||||||||||
HGF - Forschungsbereich: | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
HGF - Programm: | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
HGF - Programmthema: | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
DLR - Schwerpunkt: | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
DLR - Forschungsgebiet: | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
DLR - Teilgebiet (Projekt, Vorhaben): | keine Zuordnung | ||||||||||||||||||||||||||||||||||||
Standort: | Rhein-Sieg-Kreis | ||||||||||||||||||||||||||||||||||||
Institute & Einrichtungen: | Institut für den Schutz terrestrischer Infrastrukturen Institut für den Schutz terrestrischer Infrastrukturen > Detektionssysteme | ||||||||||||||||||||||||||||||||||||
Hinterlegt von: | Kosciow, Konrad | ||||||||||||||||||||||||||||||||||||
Hinterlegt am: | 06 Jun 2023 09:41 | ||||||||||||||||||||||||||||||||||||
Letzte Änderung: | 13 Jun 2023 09:23 |
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