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Role of DNA repair pathways in the recovery of a dried, radioresistant cyanobacterium exposed to high-LET radiation: implications for the habitability of Mars

Mosca, C. und Napoli, A. und Fagliarone, C. und Fujimori, A. und Moeller, R. und Billi, D. (2022) Role of DNA repair pathways in the recovery of a dried, radioresistant cyanobacterium exposed to high-LET radiation: implications for the habitability of Mars. International Journal of Astrobiology, 21 (5), Seiten 380-391. Cambridge University Press. doi: 10.1017/S1473550422000131. ISSN 1473-5504.

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Offizielle URL: http://www.doi.org/10.1017/S1473550422000131

Kurzfassung

If life ever appeared on Mars and if it did refuge into sub-superficial environments when surface conditions turned too hostile, then it should have been periodically revived from the frozen, dormant state in order to repair the accumulated damage and reset the survival clock to zero for the next dormant phase. Thus, unravelling how long Earth dormant microorganisms can cope with high-LET radiation mimicking long-term irradiation is fundamental to get insights into the long-term resilience of a dormant microbial life in the Martian subsurface over geological timescales that might have taken advantage of periodically clement conditions that allowed the repair of the accumulated DNA damage. The exposure of dried cells of the radioresistant cyanobacterium Chroococcidiopsis sp. CCMEE 029 to 2 kGy of heavy-ion radiation (Fe ions) did not significantly reduce its survival, although DNA damage was accumulated. Upon rehydration, DNA lesions were repaired as suggested by the over-expression of genes involved in the repair of double strand breaks (DSBs), oxidized bases and apurinic-apyrimidinic sites. Indeed, the monitoring of repair genes upon rehydration suggested a key role of the RecF homologous recombination in repairing DSBs. While the fact that out of the eight genes of the BER system, only one was up-regulated, suggested the absence of DNA lesions generally induced by UV radiation. In conclusion, the non-significantly reduced survival of dried Chroococcidiopsis exposed to 2 kGy of Fe-ion radiation further expanded our appreciation of the resilience of a putative dormant life in the Martian subsurface. Moreover, it is also relevant when searching life on Europa and Enceladus where the radiation environment might critically affect the long-term survival of dormant, frozen life forms.

elib-URL des Eintrags:https://elib.dlr.de/187622/
Dokumentart:Zeitschriftenbeitrag
Titel:Role of DNA repair pathways in the recovery of a dried, radioresistant cyanobacterium exposed to high-LET radiation: implications for the habitability of Mars
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iD
Mosca, C.department of biology, laboratory of astrobiology and molecular biology of cyanobacteria from extreme environments, university of rome tor vergata, rome, italy.
Napoli, A.Department of Biology, University of Rome Tor Vergata, Rome, Italy
Fagliarone, C.Department of Biology, Laboratory of Astrobiology and Molecular Biology of Cyanobacteria from Extreme Environments, University of Rome Tor Vergata, Rome, Italy.
Fujimori, A.National Institutes for Quantum and Radiological Science and Technology (NIRS/QST), Department of Basic Medical Sciences for Radiation Damages, Chiba, Japan.
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Billi, D.Department of Biology, University of Rome "Tor Vergata," Rome, Italy
Datum:21 April 2022
Erschienen in:International Journal of Astrobiology
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:21
DOI:10.1017/S1473550422000131
Seitenbereich:Seiten 380-391
Verlag:Cambridge University Press
ISSN:1473-5504
Status:veröffentlicht
Stichwörter:DNA damage repair; Mars habitability; high LET radiation; Chroococcidiopsis
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Projekt ISS LIFE 2.0
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin
Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Schrage, Larissa
Hinterlegt am:15 Aug 2022 12:32
Letzte Änderung:21 Mär 2023 09:56

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