elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Datenschutz | Kontakt | English
Schriftgröße: [-] Text [+]

A Meta-Analysis of the Effects of High-LET Ionizing Radiations in Human Gene Expression

Michalettou, Theodora-Dafni und Michalopoulos, Ioannis und Costes, Sylvain V. und Hellweg, Christine E. und Hada, Megumi und Georgakilas, Alexandros G. (2021) A Meta-Analysis of the Effects of High-LET Ionizing Radiations in Human Gene Expression. Life, 11 (2), Seite 115. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/life11020115. ISSN 2075-1729.

[img] PDF - Verlagsversion (veröffentlichte Fassung)
3MB

Offizielle URL: http://dx.doi.org/10.3390/life11020115

Kurzfassung

The use of high linear energy transfer (LET) ionizing radiation (IR) is progressively being incorporated in radiation therapy due to its precise dose localization and high relative biological effectiveness. At the same time, these benefits of particle radiation become a high risk for astronauts in the case of inevitable cosmic radiation exposure. Nonetheless, DNA Damage Response (DDR) activated via complex DNA damage in healthy tissue, occurring from such types of radiation, may be instrumental in the induction of various chronic and late effects. An approach to elucidating the possible underlying mechanisms is studying alterations in gene expression. To this end, we identified differentially expressed genes (DEGs) in high Z and high energy (HZE) particle-, γ-ray- and X-rayexposed healthy human tissues, utilizing microarray data available in public repositories. Differential gene expression analysis (DGEA) was conducted using the R programming language. Consequently, four separate meta-analyses were conducted, after DEG lists were grouped depending on radiation type, radiation dose and time of collection post-irradiation. To highlight the biological background of each meta-analysis group, functional enrichment analysis and biological network construction were conducted. For HZE particle exposure at 8–24 h post-irradiation, the most interesting finding is the variety of DNA repair mechanisms that were downregulated, a fact that is probably correlated with complex DNA damage formation. Simultaneously, after X-ray exposure during the same hours after irradiation, DNA repair mechanisms continue to take place. Finally, in a further comparison of lowand high-LET radiation effects, the most prominent result is that autophagy mechanisms seem to persist and that adaptive immune induction seems to be present. Such bioinformatics approaches may aid in obtaining an overview of the cellular response to high-LET particles. Understanding these response mechanisms can consequently aid in the development of countermeasures for future space missions and ameliorate heavy ion treatments.

elib-URL des Eintrags:https://elib.dlr.de/140921/
Dokumentart:Zeitschriftenbeitrag
Titel:A Meta-Analysis of the Effects of High-LET Ionizing Radiations in Human Gene Expression
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Michalettou, Theodora-DafniDNA Damage Laboratory, Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens and Centre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Greece; daphnelettos (at) biol.uoa.grhttps://orcid.org/0000-0003-2119-1099NICHT SPEZIFIZIERT
Michalopoulos, IoannisCentre of Systems Biology, Biomedical Research Foundation, Academy of Athens, 115 27 Athens, Greece; imichalop (at) bioacademy.grhttps://orcid.org/0000-0001-8991-8712NICHT SPEZIFIZIERT
Costes, Sylvain V.NASA Ames Research Center, Space Biosciences, Moffett Field, CA 94035, USA; sylvain.v.costes (at) nasa.govhttps://orcid.org/0000-0002-8542-2389NICHT SPEZIFIZIERT
Hellweg, Christine E.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Höhe, Cologne, Germany; Christine.Hellweg (at) dlr.dehttps://orcid.org/0000-0002-2223-3580NICHT SPEZIFIZIERT
Hada, MegumiRadiation Institute for Science & Engineering, Prairie View A&M University, Prairie View, TX 77446, USA; mehada (at) pvamu.eduhttps://orcid.org/0000-0002-8523-9111NICHT SPEZIFIZIERT
Georgakilas, Alexandros G.DNA Damage Laboratory, Physics Department, School of Applied Mathematical and Physical Sciences, National Technical University of Athens, 157 80 Athens, Greece;alexg (at) mail.ntua.grhttps://orcid.org/0000-0002-5971-0010NICHT SPEZIFIZIERT
Datum:3 Februar 2021
Erschienen in:Life
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:11
DOI:10.3390/life11020115
Seitenbereich:Seite 115
Verlag:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2075-1729
Status:veröffentlicht
Stichwörter:microarrays; high-LET; space radiation; differential gene expression; meta-analysis; computational radiobiology; DNA damage response; functional enrichment analysis
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Projekt ISS LIFE 2.0
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:18 Feb 2021 15:55
Letzte Änderung:14 Apr 2021 14:30

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Gestaltung Webseite und Datenbank: Copyright © Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.