Michalettou, Theodora-Dafni und Michalopoulos, Ioannis und Costes, Sylvain V. und Hellweg, Christine E. und Hada, Megumi und Georgakilas, Alexandros G. (2021) A Meta-Analysis of the Effects of High-LET Ionizing Radiations in Human Gene Expression. Life, 11 (2), Seite 115. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/life11020115. ISSN 2075-1729.
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Offizielle URL: http://dx.doi.org/10.3390/life11020115
Kurzfassung
The use of high linear energy transfer (LET) ionizing radiation (IR) is progressively being incorporated in radiation therapy due to its precise dose localization and high relative biological effectiveness. At the same time, these benefits of particle radiation become a high risk for astronauts in the case of inevitable cosmic radiation exposure. Nonetheless, DNA Damage Response (DDR) activated via complex DNA damage in healthy tissue, occurring from such types of radiation, may be instrumental in the induction of various chronic and late effects. An approach to elucidating the possible underlying mechanisms is studying alterations in gene expression. To this end, we identified differentially expressed genes (DEGs) in high Z and high energy (HZE) particle-, γ-ray- and X-rayexposed healthy human tissues, utilizing microarray data available in public repositories. Differential gene expression analysis (DGEA) was conducted using the R programming language. Consequently, four separate meta-analyses were conducted, after DEG lists were grouped depending on radiation type, radiation dose and time of collection post-irradiation. To highlight the biological background of each meta-analysis group, functional enrichment analysis and biological network construction were conducted. For HZE particle exposure at 8–24 h post-irradiation, the most interesting finding is the variety of DNA repair mechanisms that were downregulated, a fact that is probably correlated with complex DNA damage formation. Simultaneously, after X-ray exposure during the same hours after irradiation, DNA repair mechanisms continue to take place. Finally, in a further comparison of lowand high-LET radiation effects, the most prominent result is that autophagy mechanisms seem to persist and that adaptive immune induction seems to be present. Such bioinformatics approaches may aid in obtaining an overview of the cellular response to high-LET particles. Understanding these response mechanisms can consequently aid in the development of countermeasures for future space missions and ameliorate heavy ion treatments.
elib-URL des Eintrags: | https://elib.dlr.de/140921/ |
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Dokumentart: | Zeitschriftenbeitrag |
Titel: | A Meta-Analysis of the Effects of High-LET Ionizing Radiations in Human Gene Expression |
Autoren: | |
Datum: | 3 Februar 2021 |
Erschienen in: | Life |
Referierte Publikation: | Ja |
Open Access: | Ja |
Gold Open Access: | Ja |
In SCOPUS: | Ja |
In ISI Web of Science: | Ja |
Band: | 11 |
DOI: | 10.3390/life11020115 |
Seitenbereich: | Seite 115 |
Verlag: | Multidisciplinary Digital Publishing Institute (MDPI) |
ISSN: | 2075-1729 |
Status: | veröffentlicht |
Stichwörter: | microarrays; high-LET; space radiation; differential gene expression; meta-analysis; computational radiobiology; DNA damage response; functional enrichment analysis |
HGF - Forschungsbereich: | Luftfahrt, Raumfahrt und Verkehr |
HGF - Programm: | Raumfahrt |
HGF - Programmthema: | Forschung unter Weltraumbedingungen |
DLR - Schwerpunkt: | Raumfahrt |
DLR - Forschungsgebiet: | R FR - Forschung unter Weltraumbedingungen |
DLR - Teilgebiet (Projekt, Vorhaben): | R - Projekt ISS LIFE 2.0 |
Standort: | Köln-Porz |
Institute & Einrichtungen: | Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie |
Hinterlegt von: | Kopp, Kerstin |
Hinterlegt am: | 18 Feb 2021 15:55 |
Letzte Änderung: | 14 Apr 2021 14:30 |
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