elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Contact | Deutsch
Fontsize: [-] Text [+]

Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space

Ma, Xiao and Pietsch, Jessica and Wehland, Markus and Schulz, Herbert and Saar, Katrin and Hübner, Norbert and Bauer, Johann and Braun, Markus and Schwarzwälder, Achim and Segerer, Jürgen and Birlem, Maria and Horn, Astrid and Hemmersbach, Ruth and Waßer, Kai and Grosse, Jirka and Infanger, Manfred and Grimm, Daniela (2013) Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space. FASEB JOURNAL. ISSN 0892-6638.

Full text not available from this repository.

Abstract

This study focuses on the effects of short-term [22 s, parabolic flight campaign (PFC)] and long-term (10 d, Shenzhou 8 space mission) real microgravity on changes in cytokine secretion and gene expression patterns in poorly differentiated thyroid cancer cells. FTC-133 cells were cultured in space and on a random positioning machine (RPM) for 10 d, to evaluate differences between real and simulated microgravity. Multianalyte profiling was used to evaluate 128 secreted cytokines. Microarray analysis revealed 63 significantly regulated transcripts after 22 s of microgravity during a PFC and 2881 after 10 d on the RPM or in space. Genes in several biological processes, including apoptosis (n=182), cytoskeleton (n=80), adhesion/extracellular matrix (n=98), proliferation (n=184), stress response (n=268), migration (n=63), angiogenesis (n=39), and signal transduction (n=429), were differentially expressed. Genes and proteins involved in the regulation of cancer cell proliferation and metastasis, such as IL6, IL8, IL15, OPN, VEGFA, VEGFD, FGF17, MMP2, MMP3, TIMP1, PRKAA, and PRKACA, were similarly regulated under RPM and spaceflight conditions. The resulting effect was mostly antiproliferative. Gene expression during the PFC was often regulated in the opposite direction. In summary, microgravity is an invaluable tool for exploring new targets in anticancer therapy and can be simulated in some aspects in ground-based facilities.

Document Type:Article
Title:Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space
Authors:
AuthorsInstitution or Email of Authors
Ma, XiaoAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, DK-8000 Aarhus C, Denmark
Pietsch, JessicaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germany
Schulz, HerbertMax-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany
Saar, KatrinMax-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany
Hübner, NorbertMax-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, Germany
Braun, MarkusUniversity of Bonn, Institute of Molecular Physiology and Biotechnology of Plants, Bonn, Germany; mbraun@uni-bonn.de
Schwarzwälder, AchimEADS Astrium, Friedrichshafen, Germany
Segerer, JürgenEADS Astrium, Friedrichshafen, Germany
Birlem, MariaEADS Astrium, Friedrichshafen, Germany
Horn, AstridEADS Astrium, Friedrichshafen, Germany
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Waßer, KaiGerman Aerospace Center, Institute of Aerospace Medicine, Biomedical Science Support Center, Linder Hoehe, 51147 Cologne, Germany
Grosse, JirkaUniversity of Regensburg, Department of Nuclear Medicine, Franz-Josef-Strauss-allee 11, 93053 Regensburg, Germany
Infanger, ManfredCentre Hospitalier de Luxembourg, Luxembourg
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, Denmark
Date:6 November 2013
Journal or Publication Title:FASEB JOURNAL
Refereed publication:Yes
In Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
ISSN:0892-6638
Status:Published
Keywords:extracellular matrix, growth factors, microgravity, protein kinases
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Birgit Bromeis
Deposited On:10 Feb 2014 09:13
Last Modified:10 Feb 2014 09:13

Repository Staff Only: item control page

Browse
Search
Help & Contact
Informationen
electronic library is running on EPrints 3.3.12
Copyright © 2008-2012 German Aerospace Center (DLR). All rights reserved.