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Effects of ionizing radiation on human adipose derived mesenchymal stem cells and their differentiation towards the osteoblastic lineage

Konda, Bikash (2013) Effects of ionizing radiation on human adipose derived mesenchymal stem cells and their differentiation towards the osteoblastic lineage. Master's, University of Applied Sciences Bonn-Rhein-Sieg, Rheinbach..

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Abstract

Space travel presents many challenges to human health, including radiation exposure and musculoskeletal disuse. In addition, astronauts lose calcium from bones due to the uncoupling of bone formation and bone resorption. Bone forming osteoblasts are derived from undifferentiated MSCs. In this study, the ability of human adipose tissue derived stem cells (ATSC) to differentiate into the osteoblastic lineage was examined after radiation exposure as well as supplementation with osteogenic medium additives. After exposure to ionizing radiation changes in cellular morphology, cell cycle progression, as well as cellular radiosensitivity was characterized. Exposure to ionizing radiation resulted in an accumulation of cells in the G2/M phase of the cell cycle, concerning both cell lines. Alizarin Red S staining as well as quantitative determination of bone cell differentiation was performed by analyzing the hydroxyapatite content of the ECM. The results demonstrated LW24 deposited less calcium compared to SAOS-2. Additionally, gene expression analysis during differentiation process revealed expression of genes that are required for skeletal development, bone mineral metabolism and transcription factors. Detailed investigation of stem cell differentiation after radiation exposure is required to assure health of astronauts in upcoming space missions.

Document Type:Thesis (Master's)
Title:Effects of ionizing radiation on human adipose derived mesenchymal stem cells and their differentiation towards the osteoblastic lineage
Authors:
AuthorsInstitution or Email of Authors
Konda, Bikash Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Date:2013
Refereed publication:Yes
Number of Pages:86
Status:Published
Keywords:ionizing radiation, human adipose tissue derived stem cells (ATSC), osteoblastic lineage
Institution:University of Applied Sciences Bonn-Rhein-Sieg, Rheinbach.
Department:Biomedical Sciences
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:17 May 2013 09:06
Last Modified:17 May 2013 09:06

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