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Roles of the Major, Small, Acid-Soluble Spore Proteins and Spore-Specific and Universal DNA Repair Mechanisms in Resistance of Bacillus subtilis Spores to Ionizing Radiation from X Rays and High-Energy Charged-Particle Bombardment

Moeller, Ralf and Setlow, Peter and Horneck, Gerda and Berger, Thomas and Reitz, Günther and Rettberg, Petra and Doherty, Aidan J. and Okayasu, Ryuichi and Nicholson, Wayne L. (2008) Roles of the Major, Small, Acid-Soluble Spore Proteins and Spore-Specific and Universal DNA Repair Mechanisms in Resistance of Bacillus subtilis Spores to Ionizing Radiation from X Rays and High-Energy Charged-Particle Bombardment. JOURNAL OF BACTERIOLOGY, 190 (3), pp. 1134-1140. American Society for Microbiology. DOI: doi:10.1128/JB.01644-07.

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Abstract

The role of DNA repair by nonhomologous end joining (NHEJ), homologous recombination, spore photoproduct lyase, and DNA polymerase I and genome protection via α/β-type small, acid-soluble spore proteins (SASP) in Bacillus subtilis spore resistance to accelerated heavy ions (high-energy charged [HZE] particles) and X rays has been studied. Spores deficient in NHEJ and α/β-type SASP were significantly more sensitive to HZE particle bombardment and X-ray irradiation than were the recA, polA, and splB mutant and wild-type spores, indicating that NHEJ provides an efficient DNA double-strand break repair pathway during spore germination and that the loss of the α/β-type SASP leads to a significant radiosensitivity to ionizing radiation, suggesting the essential function of these spore proteins as protectants of spore DNA against ionizing radiation.

Document Type:Article
Title:Roles of the Major, Small, Acid-Soluble Spore Proteins and Spore-Specific and Universal DNA Repair Mechanisms in Resistance of Bacillus subtilis Spores to Ionizing Radiation from X Rays and High-Energy Charged-Particle Bombardment
Authors:
AuthorsInstitution or Email of Authors
Moeller, RalfUNSPECIFIED
Setlow, PeterDepartment of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut
Horneck, GerdaUNSPECIFIED
Berger, ThomasUNSPECIFIED
Reitz, GüntherUNSPECIFIED
Rettberg, PetraUNSPECIFIED
Doherty, Aidan J.Genome Damage and Stability Centre, University of Sussex, Brighton BN1, United Kingdom
Okayasu, RyuichiInternational Space Radiation Laboratory, National Institute of Radiological Sciences, Chiba-shi, Japan
Nicholson, Wayne L.University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida
Date:2008
Journal or Publication Title:JOURNAL OF BACTERIOLOGY
Refereed publication:Yes
In ISI Web of Science:Yes
Volume:190
DOI:doi:10.1128/JB.01644-07
Page Range:pp. 1134-1140
Publisher:American Society for Microbiology
Status:Published
Keywords:Bacillus subtilis, HZE, X-Rays, DNA repair
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:19 Jun 2008
Last Modified:27 Apr 2009 14:58

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