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Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission

Billi, D. and Verseux, C. and Rabbow, Elke and Rettberg, Petra (2017) Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission. EANA 2017, 14.-17. August, 2017, Aarhus, Denmark.

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Abstract

The aim of the experiment Biofilm Organisms Surfing Space (BOSS), part of the EXPOSE- R2 space mission, is to test whether biofilms can withstand long-term exposure to space and Martian conditions than planktonic counterpart. Three desert isolates of Chroococcidiopsis spp. were included in the BOSS experiment: strain CCMEE 029 from endolithic commu- nities in the Negev Desert, and strains CCMEE 057 and CCMEE 064 from endolithic and hypolithic communities in the Sinai Desert. Dried biofilms and dried multilayered planktonic samples were exposed to both ground-based simulations and to space and Martian simulated conditions in Low Earth Orbit (LEO) within the ESA facility EXPOSE-R2 outside the Inter- national Space Station. Samples were exposed for 16 months to space and Martian simulated conditions, characterized by temperature variations, ionizing radiation, vacuum or simulated Martian atmosphere, in the dark or under attenuated space and Mars-like and solar UV ir- radiation. The effects of those environments on cyanobacterial samples were investigated by using confocal laser scanning microscopy to visualize the biofilm architecture and quantify photosynthetic pigment autofluorescence, PCR-based assays to asses DNA damage and and colony forming ability to test the recovery upon rehydration. Results from the flight mission are consistent with previous ground-based simulations of the mission1,2 and demonstrate an overall higher resistance of biofilms when compared to the planktonic counterpart, showing the former an increased viability and lower amounts of DNA damage.

Item URL in elib:https://elib.dlr.de/114340/
Document Type:Conference or Workshop Item (Speech)
Title:Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Billi, D.Department of Biology ,University of Rome “Tor Vergata”, Department of Biology, Rome, Italy.UNSPECIFIED
Verseux, C.Department of Biology ,University of Rome “Tor Vergata”, Department of Biology, Rome, Italy.UNSPECIFIED
Rabbow, Elkeradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; elke.rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Rettberg, Petraradiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Date:2017
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:EXPOSE-R2 space mission
Event Title:EANA 2017
Event Location:Aarhus, Denmark
Event Type:international Conference
Event Dates:14.-17. August, 2017
Organizer:European Astrobiology Network Association
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:25 Sep 2017 13:58
Last Modified:31 Jul 2019 20:11

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