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Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission

Billi, D. und Verseux, C. und Rabbow, E. und Rettberg, P. (2017) Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission. EANA 2017, 14.-17. August, 2017, Aarhus, Denmark.

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Kurzfassung

The aim of the experiment Biofilm Organisms Surfing Space (BOSS), part of the EXPOSE- R2 space mission, is to test whether biofilms can withstand long-term exposure to space and Martian conditions than planktonic counterpart. Three desert isolates of Chroococcidiopsis spp. were included in the BOSS experiment: strain CCMEE 029 from endolithic commu- nities in the Negev Desert, and strains CCMEE 057 and CCMEE 064 from endolithic and hypolithic communities in the Sinai Desert. Dried biofilms and dried multilayered planktonic samples were exposed to both ground-based simulations and to space and Martian simulated conditions in Low Earth Orbit (LEO) within the ESA facility EXPOSE-R2 outside the Inter- national Space Station. Samples were exposed for 16 months to space and Martian simulated conditions, characterized by temperature variations, ionizing radiation, vacuum or simulated Martian atmosphere, in the dark or under attenuated space and Mars-like and solar UV ir- radiation. The effects of those environments on cyanobacterial samples were investigated by using confocal laser scanning microscopy to visualize the biofilm architecture and quantify photosynthetic pigment autofluorescence, PCR-based assays to asses DNA damage and and colony forming ability to test the recovery upon rehydration. Results from the flight mission are consistent with previous ground-based simulations of the mission1,2 and demonstrate an overall higher resistance of biofilms when compared to the planktonic counterpart, showing the former an increased viability and lower amounts of DNA damage.

Dokumentart:Konferenzbeitrag (Vortrag)
Titel:Endurance of desert-cyanobacteria biofilms to space and simulated Mars conditions during the EXPOSE-R2 space mission
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iD
Billi, D.Department of Biology ,University of Rome “Tor Vergata”, Department of Biology, Rome, Italy.NICHT SPEZIFIZIERT
Verseux, C.Department of Biology ,University of Rome “Tor Vergata”, Department of Biology, Rome, Italy.NICHT SPEZIFIZIERT
Rabbow, E.radiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; elke.rabbow@dlr.dehttps://orcid.org/0000-0002-9301-2021
Rettberg, P.radiation biology department, institute of aerospace medicine, german aerospace center (dlr), cologne, germany; petra.rettberg@dlr.dehttps://orcid.org/0000-0003-4439-2395
Datum:2017
Referierte Publikation:Ja
In Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:EXPOSE-R2 space mission
Veranstaltungstitel:EANA 2017
Veranstaltungsort:Aarhus, Denmark
Veranstaltungsart:internationale Konferenz
Veranstaltungsdatum:14.-17. August, 2017
Veranstalter :European Astrobiology Network Association
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:25 Sep 2017 13:58
Letzte Änderung:25 Sep 2017 13:58

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