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Salt-dependent chemotaxis of macrophages

Müller, S. and Quast, T. and Schröder, A. and Hucke, S. and Klotz, L. and Jantsch, J. and Gerzer, R. and Hemmersbach, Ruth and Kolanus, Waldemar (2013) Salt-dependent chemotaxis of macrophages. PLoS One (8). DOI: 10.1371/journal.pone.0073439. ISSN 1932-6203.

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Official URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073439

Abstract

Besides their role in immune system host defense, there is growing evidence that macrophages may also be important regulators of salt homeostasis and blood pressure by a TonEBP-VEGF-C dependent buffering mechanism. As macrophages are known to accumulate in the skin of rats fed under high salt diet conditions and are pivotal for removal of high salt storage, the question arose how macrophages sense sites of high sodium storage. Interestingly, we observed that macrophage-like RAW264.7 cells, murine bone marrow-derived macrophages and peritoneal macrophages recognize NaCl hypertonicity as a chemotactic stimulus and migrate in the direction of excess salt concentration by using an in vitro transwell migration assay. While RAW264.7 cells migrated toward NaCl in a dose-dependent fashion, no migratory response toward isotonic or hypotonic media controls, or other osmo-active agents, e.g. urea or mannitol, could be detected. Interestingly, we could not establish a specific role of the osmoprotective transcription factor TonEBP in regulating salt-dependent chemotaxis, since the specific migration of bone marrow-derived macrophages following RNAi of TonEBP toward NaCl was not altered. Although the underlying mechanism remains unidentified, these data point to a thus far unappreciated role for NaCl-dependent chemotaxis of macrophages in the clearance of excess salt, and suggest the existence of novel NaCl sensor/effector circuits, which are independent of the TonEBP system.

Document Type:Article
Title:Salt-dependent chemotaxis of macrophages
Authors:
AuthorsInstitution or Email of Authors
Müller, S.Institute of Aerospace Medicine, German Aerospace Center, Cologne, Germany
Quast, T.Laboratory of Molecular Immunology, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany
Schröder, A.Interdisciplinary Center for Clinical Research, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany
Hucke, S.Clinic for Neurology – Inflammatory Disorders of the Central Nervous System and Neurooncology, University of Münster, Münster, Germany
Klotz, L.Clinic for Neurology – Inflammatory Disorders of the Central Nervous System and Neurooncology, University of Münster, Münster, Germany
Jantsch, J.Microbiology Institute – Clinical Microbiology, Immunology and Hygiene, University Hospital of Erlangen and Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany
Gerzer, R.Institute of Aerospace Medicine, German Aerospace Center, Cologne, Germany
Hemmersbach, RuthInstitute of Aerospace Medicine, German Aerospace Center, Cologne, Germany
Kolanus, WaldemarLaboratory of Molecular Immunology, Life and Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany
Date:16 September 2013
Journal or Publication Title:PLoS One
Refereed publication:Yes
In Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
DOI:10.1371/journal.pone.0073439
Editors:
EditorsEmail
Ko, Ben C. B.Chinese University of Hong Kong, Hong Kong
ISSN:1932-6203
Status:Published
Keywords:cell migration, chemokines, macrophages, transcription factors
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Silke Müller
Deposited On:10 Feb 2014 09:17
Last Modified:10 Feb 2014 09:17

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