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Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots

Aubry-Hivet, Dorothee und Nziengui, Hugues und Rapp, Katja und Oliveira, Oscar und Paponov, Ivan und Li, Yong und Hauslage, Jens und Vagt, Nicole und Braun, Markus und Ditengou, Franck Anicet und Dovzhenko, Alexander und Palme, Klaus (2014) Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots. Plant Biology, 16 (1), Seiten 129-141. DOI: 10.1111/plb.12130. ISSN 1435-8603.

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Kurzfassung

Plant roots are among most intensively studied biological systems in gravity research. Altered gravity induces asymmetric cell growth leading to root bending. Differential distribution of the phytohormone auxin underlies root responses to gravity, being coordinated by auxin efflux transporters from the PIN family. The objective of this study was to compare early transcriptomic changes in roots of Arabidopsis thaliana wild type, and pin2 and pin3 mutants under parabolic flight conditions and to correlate these changes to auxin distribution. Parabolic flights allow comparison of transient 1−g, hypergravity and microgravity effects in living organisms in parallel. We found common and mutation-related genes differentially expressed in response to transient microgravity phases. Gene ontology analysis of common genes revealed lipid metabolism, response to stress factors and light categories as primarily involved in response to transient microgravity phases, suggesting that fundamental reorganisation of metabolic pathways functions upstream of a further signal mediating hormonal network. Gene expression changes in roots lacking the columella-located PIN3 were stronger than in those deprived of the epidermis and cortex cell-specific PIN2. Moreover, repetitive exposure to microgravity/hypergravity and gravity/hypergravity flight phases induced an up-regulation of auxin responsive genes in wild type and pin2 roots, but not in pin3 roots, suggesting a critical function of PIN3 in mediating auxin fluxes in response to transient microgravity phases. Our study provides important insights towards understanding signal transduction processes in transient microgravity conditions by combining for the first time the parabolic flight platform with the transcriptome analysis of different genetic mutants in the model plant, Arabidopsis.

Dokumentart:Zeitschriftenbeitrag
Titel:Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Aubry-Hivet, DorotheeInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Nziengui, HuguesInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Rapp, KatjaInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Oliveira, OscarInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Paponov, IvanInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Li, YongCentre for Biological Systems Analysis, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, Germany
Vagt, NicoleGravitational Biology, IMBIO, University of Bonn, Germany
Braun, MarkusGravitational Biology, IMBIO, University of Bonn, Germany
Ditengou, Franck AnicetInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Dovzhenko, AlexanderInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Palme, KlausInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Datum:Januar 2014
Erschienen in:Plant Biology
Referierte Publikation:Ja
In Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:16
DOI :10.1111/plb.12130
Seitenbereich:Seiten 129-141
ISSN:1435-8603
Status:veröffentlicht
Stichwörter:Arabidopsis thaliana, microgravity, mutant, root, transcriptome
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Biomedizinisches Wissenschafts-Unterstützungszentrum
Hinterlegt von: Birgit Bromeis
Hinterlegt am:10 Feb 2014 09:14
Letzte Änderung:10 Feb 2014 09:14

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