Aubry-Hivet, Dorothee and Nziengui, Hugues and Rapp, Katja and Oliveira, Oscar and Paponov, Ivan and Li, Yong and Hauslage, Jens and Vagt, Nicole and Braun, Markus and Ditengou, Franck Anicet and Dovzhenko, Alexander and Palme, Klaus (2014) Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots. Plant Biology, 16 (1), pp. 129-141. DOI: 10.1111/plb.12130. ISSN 1435-8603.
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Plant roots are among most intensively studied biological systems in gravity research. Altered gravity induces asymmetric cell growth leading to root bending. Differential distribution of the phytohormone auxin underlies root responses to gravity, being coordinated by auxin efflux transporters from the PIN family. The objective of this study was to compare early transcriptomic changes in roots of Arabidopsis thaliana wild type, and pin2 and pin3 mutants under parabolic flight conditions and to correlate these changes to auxin distribution. Parabolic flights allow comparison of transient 1−g, hypergravity and microgravity effects in living organisms in parallel. We found common and mutation-related genes differentially expressed in response to transient microgravity phases. Gene ontology analysis of common genes revealed lipid metabolism, response to stress factors and light categories as primarily involved in response to transient microgravity phases, suggesting that fundamental reorganisation of metabolic pathways functions upstream of a further signal mediating hormonal network. Gene expression changes in roots lacking the columella-located PIN3 were stronger than in those deprived of the epidermis and cortex cell-specific PIN2. Moreover, repetitive exposure to microgravity/hypergravity and gravity/hypergravity flight phases induced an up-regulation of auxin responsive genes in wild type and pin2 roots, but not in pin3 roots, suggesting a critical function of PIN3 in mediating auxin fluxes in response to transient microgravity phases. Our study provides important insights towards understanding signal transduction processes in transient microgravity conditions by combining for the first time the parabolic flight platform with the transcriptome analysis of different genetic mutants in the model plant, Arabidopsis.
|Title:||Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots|
|Journal or Publication Title:||Plant Biology|
|In Open Access:||No|
|In ISI Web of Science:||Yes|
|Page Range:||pp. 129-141|
|Keywords:||Arabidopsis thaliana, microgravity, mutant, root, transcriptome|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Space|
|HGF - Program Themes:||Research under Space Conditions|
|DLR - Research area:||Raumfahrt|
|DLR - Program:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||R - Vorhaben Biowissenschaftliche Nutzerunterstützung|
|Institutes and Institutions:||Institute of Aerospace Medicine > Biomedical Science Support Center|
|Deposited By:||Birgit Bromeis|
|Deposited On:||10 Feb 2014 09:14|
|Last Modified:||10 Feb 2014 09:14|
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