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Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots

Aubry-Hivet, Dorothee and Nziengui, Hugues and Rapp, Katja and Oliveira, Oscar and Paponov, Ivan and Li, Yong and Hauslage, Jens and Vagt, Nicole and Braun, Markus and Ditengou, Franck Anicet and Dovzhenko, Alexander and Palme, Klaus (2014) Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots. Plant Biology, 16 (1), pp. 129-141. DOI: 10.1111/plb.12130. ISSN 1435-8603.

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Abstract

Plant roots are among most intensively studied biological systems in gravity research. Altered gravity induces asymmetric cell growth leading to root bending. Differential distribution of the phytohormone auxin underlies root responses to gravity, being coordinated by auxin efflux transporters from the PIN family. The objective of this study was to compare early transcriptomic changes in roots of Arabidopsis thaliana wild type, and pin2 and pin3 mutants under parabolic flight conditions and to correlate these changes to auxin distribution. Parabolic flights allow comparison of transient 1−g, hypergravity and microgravity effects in living organisms in parallel. We found common and mutation-related genes differentially expressed in response to transient microgravity phases. Gene ontology analysis of common genes revealed lipid metabolism, response to stress factors and light categories as primarily involved in response to transient microgravity phases, suggesting that fundamental reorganisation of metabolic pathways functions upstream of a further signal mediating hormonal network. Gene expression changes in roots lacking the columella-located PIN3 were stronger than in those deprived of the epidermis and cortex cell-specific PIN2. Moreover, repetitive exposure to microgravity/hypergravity and gravity/hypergravity flight phases induced an up-regulation of auxin responsive genes in wild type and pin2 roots, but not in pin3 roots, suggesting a critical function of PIN3 in mediating auxin fluxes in response to transient microgravity phases. Our study provides important insights towards understanding signal transduction processes in transient microgravity conditions by combining for the first time the parabolic flight platform with the transcriptome analysis of different genetic mutants in the model plant, Arabidopsis.

Document Type:Article
Title:Analysis of gene expression during parabolic flights reveals distinct early gravity responses in arabidopsis roots
Authors:
AuthorsInstitution or Email of Authors
Aubry-Hivet, DorotheeInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Nziengui, HuguesInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Rapp, KatjaInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Oliveira, OscarInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Paponov, IvanInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Li, YongCentre for Biological Systems Analysis, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Biomedical Science Support Center, Cologne, Germany
Vagt, NicoleGravitational Biology, IMBIO, University of Bonn, Germany
Braun, MarkusGravitational Biology, IMBIO, University of Bonn, Germany
Ditengou, Franck AnicetInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Dovzhenko, AlexanderInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Palme, KlausInstitute of Biology II/Botany, Faculty of Biology, Albert-Ludwigs University of Freiburg, Freiburg, Germany
Date:January 2014
Journal or Publication Title:Plant Biology
Refereed publication:Yes
In Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:16
DOI:10.1111/plb.12130
Page Range:pp. 129-141
ISSN:1435-8603
Status:Published
Keywords:Arabidopsis thaliana, microgravity, mutant, root, transcriptome
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Birgit Bromeis
Deposited On:10 Feb 2014 09:14
Last Modified:10 Feb 2014 09:14

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