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The impact of altered gravity and vibration on endothelial cells during a parabolic flight

Wehland, Markus and Ma, Xiao and Braun, Markus and Hauslage, Jens and Hemmersbach, Ruth and Bauer, Johann and Infanger, Manfred and Grimm, Daniela (2013) The impact of altered gravity and vibration on endothelial cells during a parabolic flight. Cellular Physiology and Biochemistry, 31 (2-3), pp. 432-451. DOI: 10.1159/000343380. ISSN 1015-8987.

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Endothelial cells (EC) cultured under altered gravity conditions show a cytoskeletal disorganization and differential gene expression (short-term effects), as well as apoptosis in adherently growing EC or formation of tubular 3D structures (long-term effects). Methods: Investigating short-term effects of real microgravity, we exposed EC to parabolic flight maneuvers and analysed them on both protein and transcriptional level. The effects of hypergravity and vibration were studied separately. Results: Pan-actin and tubulin proteins were elevated by vibration and down-regulated by hypergravity. beta-Actin was reduced by vibration. Moesin protein was reduced by both vibration and hypergravity, ezrin potein was strongly elevated under vibration. Gene expression of ACTB, CCND1, CDC6, CDKN1A, VEGFA, FLK-1, EZR, ITBG1, OPN, CASP3, CASP8, ANXA2, and BIRC5 was reduced under vibration. With the exception of CCNA2, CCND1, MSN, RDX, OPN, BIRC5, and ACTB all investigated genes were downregulated by hypergravity. After one parabola (P) CCNA2, CCND1, CDC6, CDKN1A, EZR, MSN, OPN, VEGFA, CASP3, CASP8, ANXA1, ANXA2, and BIRC5 were up-, while FLK1 was downregulated. EZR, MSN, OPN, ANXA2, and BIRC5 were upregulated after 31P. Conclusions: Genes of the cytoskeleton, angiogenesis, extracellular matrix, apoptosis, and cell cycle regulation were affected by parabolic flight maneuvers. We show that the microgravity stimulus is stronger than hypergravity/vibration.

Document Type:Article
Title:The impact of altered gravity and vibration on endothelial cells during a parabolic flight
AuthorsInstitution or Email of Authors
Wehland, MarkusInstitute of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin, Berlin, Germany
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark
Braun, MarkusGerman Aerospace Center (DLR), Space Administration, Bonn, Germany
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Bauer, JohannMax-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, Denmark
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
In Open Access:Yes
In ISI Web of Science:Yes
Page Range:pp. 432-451
Keywords:activated protein-kinase, fibroblast-growth-factor, simulated microgravity, cancer-cells, cylin-A, parabolic flight, extracellular-matrix, hypergravity, microgravity Vibration; Cytoskeleton; Angiogenesis; Extracellular matrix; Apoptosis; Cell cycle;
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Birgit Bromeis
Deposited On:10 Feb 2014 09:12
Last Modified:10 Feb 2014 09:12

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