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The impact of altered gravity and vibration on endothelial cells during a parabolic flight

Wehland, Markus und Ma, Xiao und Braun, Markus und Hauslage, Jens und Hemmersbach, Ruth und Bauer, Johann und Infanger, Manfred und Grimm, Daniela (2013) The impact of altered gravity and vibration on endothelial cells during a parabolic flight. Cellular Physiology and Biochemistry, 31 (2-3), Seiten 432-451. DOI: 10.1159/000343380. ISSN 1015-8987.

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Kurzfassung

Endothelial cells (EC) cultured under altered gravity conditions show a cytoskeletal disorganization and differential gene expression (short-term effects), as well as apoptosis in adherently growing EC or formation of tubular 3D structures (long-term effects). Methods: Investigating short-term effects of real microgravity, we exposed EC to parabolic flight maneuvers and analysed them on both protein and transcriptional level. The effects of hypergravity and vibration were studied separately. Results: Pan-actin and tubulin proteins were elevated by vibration and down-regulated by hypergravity. beta-Actin was reduced by vibration. Moesin protein was reduced by both vibration and hypergravity, ezrin potein was strongly elevated under vibration. Gene expression of ACTB, CCND1, CDC6, CDKN1A, VEGFA, FLK-1, EZR, ITBG1, OPN, CASP3, CASP8, ANXA2, and BIRC5 was reduced under vibration. With the exception of CCNA2, CCND1, MSN, RDX, OPN, BIRC5, and ACTB all investigated genes were downregulated by hypergravity. After one parabola (P) CCNA2, CCND1, CDC6, CDKN1A, EZR, MSN, OPN, VEGFA, CASP3, CASP8, ANXA1, ANXA2, and BIRC5 were up-, while FLK1 was downregulated. EZR, MSN, OPN, ANXA2, and BIRC5 were upregulated after 31P. Conclusions: Genes of the cytoskeleton, angiogenesis, extracellular matrix, apoptosis, and cell cycle regulation were affected by parabolic flight maneuvers. We show that the microgravity stimulus is stronger than hypergravity/vibration.

Dokumentart:Zeitschriftenbeitrag
Titel:The impact of altered gravity and vibration on endothelial cells during a parabolic flight
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Wehland, MarkusInstitute of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin, Berlin, Germany
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark
Braun, MarkusGerman Aerospace Center (DLR), Space Administration, Bonn, Germany
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Bauer, JohannMax-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
Grimm, DanielaAarhus University, Institute of Biomedicine, Pharmacology, Wilhelm Meyers Allé 4, 8000 Aarhus C, Denmark
Datum:2013
Erschienen in:Cellular Physiology and Biochemistry
Referierte Publikation:Ja
In Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:31
DOI :10.1159/000343380
Seitenbereich:Seiten 432-451
ISSN:1015-8987
Status:veröffentlicht
Stichwörter:activated protein-kinase, fibroblast-growth-factor, simulated microgravity, cancer-cells, cylin-A, parabolic flight, extracellular-matrix, hypergravity, microgravity Vibration; Cytoskeleton; Angiogenesis; Extracellular matrix; Apoptosis; Cell cycle;
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Biomedizinisches Wissenschafts-Unterstützungszentrum
Hinterlegt von: Birgit Bromeis
Hinterlegt am:10 Feb 2014 09:12
Letzte Änderung:10 Feb 2014 09:12

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