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Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells

Ma, Xiao und Wehland, Markus und Aleshcheva, Ganna und Hauslage, Jens und Waßer, Kai und Hemmersbach, Ruth und Infanger, Manfred und Bauer, Johann und Grimm, Daniela (2013) Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells. PLoS One, 8 (7). DOI: 10.1371/journal.pone.0068140. ISSN 1932-6203.

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Kurzfassung

It is known that exposing cell lines in vitro to parabolic flights changes their gene expression and protein production patterns. Parabolic flights and spaceflight in general are accompanied by transient hypergravity and vibration, which may impact the cells and therefore, have to be considered too. To estimate the possible impact of transient hypergravity and vibration, we investigated the effects of these forces separately using dedicated ground-based facilities. We placed follicular thyroid ML-1 and CGTH W-1 cancer cells in a specific centrifuge (MuSIC Multi Sample Incubator Centrifuge; SAHC Short Arm Human Centrifuge) simulating the hypergravity phases that occur during one (P1) and 31 parabolas (P31) of parabolic flights, respectively. On the Vibraplex device, the same cell lines were treated with vibration waves corresponding to those that occur during a whole parabolic flight lasting for two hours. After the various treatments, cells were harvested and analyzed by quantitative real-time PCR, focusing on the genes involved in forming (ACTB, MYO9, TUBB, VIM, TLN1, and ITGB1) and modulating (EZR, RDX, and MSN) the cytoskeleton, as well as those encoding growth factors (EGF, CTGF, IL6, and IL8) or protein kinases (PRKAA1 and PRKCA). The analysis revealed alterations in several genes in both cell lines; however, fewer genes were affected in ML-1 than CGTH W-1 cells. Interestingly, IL6 was the only gene whose expression was changed in both cell lines by each treatment, while PKCA transcription remained unaffected in all experiments. We conclude that a PKCa-independent mechanism of IL6 gene activation is very sensitive to physical forces in thyroid cells cultured in vitro as monolayers.

Dokumentart:Zeitschriftenbeitrag
Titel:Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, DenmarkNICHT SPEZIFIZIERT
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyNICHT SPEZIFIZIERT
Aleshcheva, GannaOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyNICHT SPEZIFIZIERT
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyNICHT SPEZIFIZIERT
Waßer, KaiGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyNICHT SPEZIFIZIERT
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, GermanyNICHT SPEZIFIZIERT
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyNICHT SPEZIFIZIERT
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, GermanyNICHT SPEZIFIZIERT
Grimm, DanielaInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, DenmarkNICHT SPEZIFIZIERT
Datum:2 Juli 2013
Erschienen in:PLoS One
Referierte Publikation:Ja
In Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:8
DOI :10.1371/journal.pone.0068140
ISSN:1932-6203
Status:veröffentlicht
Stichwörter:Interleukin-6, Thyroid gland, Cancer, gene expression, cellELL lines, stress (pysiology), cytoskeletal proteins
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Biomedizinisches Wissenschafts-Unterstützungszentrum
Hinterlegt von: Bromeis, Birgit
Hinterlegt am:10 Feb 2014 09:11
Letzte Änderung:10 Feb 2014 09:11

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