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Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells

Ma, Xiao and Wehland, Markus and Aleshcheva, Ganna and Hauslage, Jens and Waßer, Kai and Hemmersbach, Ruth and Infanger, Manfred and Bauer, Johann and Grimm, Daniela (2013) Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells. PLoS One, 8 (7). DOI: 10.1371/journal.pone.0068140. ISSN 1932-6203.

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Abstract

It is known that exposing cell lines in vitro to parabolic flights changes their gene expression and protein production patterns. Parabolic flights and spaceflight in general are accompanied by transient hypergravity and vibration, which may impact the cells and therefore, have to be considered too. To estimate the possible impact of transient hypergravity and vibration, we investigated the effects of these forces separately using dedicated ground-based facilities. We placed follicular thyroid ML-1 and CGTH W-1 cancer cells in a specific centrifuge (MuSIC Multi Sample Incubator Centrifuge; SAHC Short Arm Human Centrifuge) simulating the hypergravity phases that occur during one (P1) and 31 parabolas (P31) of parabolic flights, respectively. On the Vibraplex device, the same cell lines were treated with vibration waves corresponding to those that occur during a whole parabolic flight lasting for two hours. After the various treatments, cells were harvested and analyzed by quantitative real-time PCR, focusing on the genes involved in forming (ACTB, MYO9, TUBB, VIM, TLN1, and ITGB1) and modulating (EZR, RDX, and MSN) the cytoskeleton, as well as those encoding growth factors (EGF, CTGF, IL6, and IL8) or protein kinases (PRKAA1 and PRKCA). The analysis revealed alterations in several genes in both cell lines; however, fewer genes were affected in ML-1 than CGTH W-1 cells. Interestingly, IL6 was the only gene whose expression was changed in both cell lines by each treatment, while PKCA transcription remained unaffected in all experiments. We conclude that a PKCa-independent mechanism of IL6 gene activation is very sensitive to physical forces in thyroid cells cultured in vitro as monolayers.

Document Type:Article
Title:Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells
Authors:
AuthorsInstitution or Email of Authors
Ma, XiaoInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark
Wehland, MarkusOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germany
Aleshcheva, GannaOtto-von-Guericke-University Magdeburg, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germany
Hauslage, JensGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Waßer, KaiGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Cologne, Germany
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
Bauer, JohannMax-Planck Institute for Biochemistry, Martinsried, Germany
Grimm, DanielaInstitute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark
Date:2 July 2013
Journal or Publication Title:PLoS One
Refereed publication:Yes
In Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:8
DOI:10.1371/journal.pone.0068140
ISSN:1932-6203
Status:Published
Keywords:Interleukin-6, Thyroid gland, Cancer, gene expression, cellELL lines, stress (pysiology), cytoskeletal proteins
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Birgit Bromeis
Deposited On:10 Feb 2014 09:11
Last Modified:10 Feb 2014 09:11

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