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Biofilm and Planktonic Lifestyles Differently Support the Resistance of the Desert Cyanobacterium Chroococcidiopsis Under Space and Martian Simulations

Baqué, Mickael and Scalzi, Giuliano and Rabbow, Elke and Rettberg, Petra and Billi, Daniela (2013) Biofilm and Planktonic Lifestyles Differently Support the Resistance of the Desert Cyanobacterium Chroococcidiopsis Under Space and Martian Simulations. Origins of Life and Evolution of Biospheres, Epub ahead of print (In Press). Springer Science+Business Media Dordrecht . DOI: 10.1007/s11084-013-9341-6. ISSN 0169-6149.

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Abstract

When Chroococcidiopsis sp. strain CCMEE 057 from the Sinai Desert and strain CCMEE 029 from the Negev Desert were exposed to space and Martian simulations in the dried status as biofilms or multilayered planktonic samples, the biofilms exhibited an enhanced rate of survival. Compared to strain CCMEE 029, biofilms of strain CCME 057 better tolerated UV polychromatic radiation (5 × 10⁵ kJ/m² attenuated with a 0.1 % neutral density filter) combined with space vacuum or Martian atmosphere of 780 Pa. CCMEE 029, on the other hand, failed to survive UV polychromatic doses higher than 1.5 × 10³ kJ/m². The induced damage to genomic DNA, plasma membranes and photosynthetic apparatus was quantified and visualized by means of PCR-based assays and CLSM imaging. Planktonic samples of both strains accumulated a higher amount of damage than did the biofilms after exposure to each simulation; CLSM imaging showed that photosynthetic pigment bleaching, DNA fragmentation and damaged plasma membranes occurred in the top 3–4 cell layers of both biofilms and of multilayered planktonic samples. Differences in the EPS composition were revealed by molecular probe staining as contributing to the enhanced endurance of biofilms compared to that of planktonic samples. Our results suggest that compared to strain CCMEE 029, biofilms of strain CCMEE 057 might better tolerate 1 year’s exposure in space during the next EXPOSE-R2 mission.

Document Type:Article
Title:Biofilm and Planktonic Lifestyles Differently Support the Resistance of the Desert Cyanobacterium Chroococcidiopsis Under Space and Martian Simulations
Authors:
AuthorsInstitution or Email of Authors
Baqué, Mickael Dipartimento di Biologia, Università di Roma “Tor Vergata”, Rome, Italy
Scalzi, Giuliano Dipartimento di Biologia, Università di Roma “Tor Vergata”, Rome, Italy
Rabbow, Elke Institute of Aerospace Medicine, DLR, Cologne, Germany
Rettberg, Petra Institute of Aerospace Medicine, DLR, Cologne, Germany
Billi, Daniela Dipartimento di Biologia, Università di Roma “Tor Vergata”, Rome, Italy, e-mail: billi@uniroma2.it
Date:2013
Journal or Publication Title:Origins of Life and Evolution of Biospheres
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:Epub ahead of print (In Press)
DOI:10.1007/s11084-013-9341-6
Publisher:Springer Science+Business Media Dordrecht
ISSN:0169-6149
Status:Published
Keywords:Space simulation, Mars simulation, Astrobiology, Chroococcidiopsis, Biofilm, DNA damage
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:12 Sep 2013 13:47
Last Modified:12 Sep 2013 13:47

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