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Utilization of Low-Pressure Plasma to Inactivate Bacterial Spores on Stainless Steel Screws

Stapelmann, Katharina und Fiebrandt, Marcel und Raguse, Marina und Awakowicz, Peter und Reitz, Günther und Moeller, Ralf (2013) Utilization of Low-Pressure Plasma to Inactivate Bacterial Spores on Stainless Steel Screws. Astrobiology, 13 (7), Seiten 597-606. Mary Ann Liebert, Inc.. DOI: 10.1089/ast.2012.0949. ISSN 1531-1074.

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Kurzfassung

A special focus area of planetary protection is the monitoring, control, and reduction of microbial contaminations that are detected on spacecraft components and hardware during and after assembly. In this study, wildtype spores of Bacillus pumilus SAFR-032 (a persistent spacecraft assembly facility isolate) and the laboratory model organism B. subtilis 168 were used to study the effects of low-pressure plasma, with hydrogen alone and in combination with oxygen and evaporated hydrogen peroxide as a process gas, on spore survival, which was determined by a colony formation assay. Spores of B. pumilus SAFR-032 and B. subtilis 168 were deposited with an aseptic technique onto the surface of stainless steel screws to simulate a spore-contaminated spacecraft hardware component, and were subsequently exposed to different plasmas and hydrogen peroxide conditions in a very high frequency capacitively coupled plasma reactor (VHF-CCP) to reduce the spore burden. Spores of the spacecraft isolate B. pumilus SAFR-032 were significantly more resistant to plasma treatment than spores of B. subtilis 168. The use of low-pressure plasma with an additional treatment of evaporated hydrogen peroxide also led to an enhanced spore inactivation that surpassed either single treatment when applied alone, which indicates the potential application of this method as a fast and suitable way to reduce spore-contaminated spacecraft hardware components for planetary protection purposes.

Dokumentart:Zeitschriftenbeitrag
Titel:Utilization of Low-Pressure Plasma to Inactivate Bacterial Spores on Stainless Steel Screws
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Stapelmann, Katharina Ruhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, Germany.
Fiebrandt, Marcel Ruhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, Germany.
Raguse, Marina German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), Germany.
Awakowicz, Peter Ruhr University Bochum (RUB), Institute for Electrical Engineering and Plasma Technology (AEPT), Bochum, Germany.
Reitz, Günther German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), Germany.
Moeller, Ralf German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Department, Cologne (Köln), Germany.
Datum:2013
Erschienen in:Astrobiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:13
DOI :10.1089/ast.2012.0949
Seitenbereich:Seiten 597-606
Verlag:Mary Ann Liebert, Inc.
ISSN:1531-1074
Status:veröffentlicht
Stichwörter:Bacillus spores, Contamination, Spacecraft hardware, Plasma sterilization, Planetary protection
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:26 Aug 2013 12:00
Letzte Änderung:26 Aug 2013 12:00

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