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Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation

Horikawa, Daiki D. und Cumbers, John und Sakakibara, Iori und Rogoff, Dana und Leuko, Stefan und Harnoto, Raechel und Arakawa, Kazuharu und Katayama, Toshiaki und Kunieda, Takekazu und Toyoda, Atsushi und Fujiyama, Asao und Rothschild, Lynn J. (2013) Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation. PLoS One, 8 (6: e64793). PLOS. DOI: 10.1371/journal.pone.0064793. ISSN 1932-6203.

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Kurzfassung

Tardigrades inhabiting terrestrial environments exhibit extraordinary resistance to ionizing radiation and UV radiation although little is known about the mechanisms underlying the resistance. We found that the terrestrial tardigrade Ramazzottius varieornatus is able to tolerate massive doses of UVC irradiation by both being protected from forming UVCinduced thymine dimers in DNA in a desiccated, anhydrobiotic state as well as repairing the dimers that do form in the hydrated animals. In R. varieornatus accumulation of thymine dimers in DNA induced by irradiation with 2.5 kJ/m² of UVC radiation disappeared 18 h after the exposure when the animals were exposed to fluorescent light but not in the dark. Much higher UV radiation tolerance was observed in desiccated anhydrobiotic R. varieornatus compared to hydrated specimens of this species. On the other hand, the freshwater tardigrade species Hypsibius dujardini that was used as control, showed much weaker tolerance to UVC radiation than R. varieornatus, and it did not contain a putative phrA gene sequence. The anhydrobiotes of R. varieornatus accumulated much less UVC-induced thymine dimers in DNA than hydrated one. It suggests that anhydrobiosis efficiently avoids DNA damage accumulation in R. varieornatus and confers better UV radiation tolerance on this species. Thus we propose that UV radiation tolerance in tardigrades is due to the both high capacities of DNA damage repair and DNA protection, a two-pronged survival strategy.

Dokumentart:Zeitschriftenbeitrag
Titel:Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID
Horikawa, Daiki D. Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and NASA Astrobiology InstituteNICHT SPEZIFIZIERT
Cumbers, John Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, United States of AmericaNICHT SPEZIFIZIERT
Sakakibara, Iori INSERM U1016, Institut Cochin, Paris, France and CNRS UMR 8104, Paris, France and Université Paris Descartes, Sorbonne Paris Cité , Paris, France NICHT SPEZIFIZIERT
Rogoff, Dana Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of AmericaNICHT SPEZIFIZIERT
Leuko, Stefan Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Linder Hoehe, Cologne (Koeln), GermanyNICHT SPEZIFIZIERT
Harnoto, Raechel California Polytechnic State University, San Luis Obispo, California, United States of AmericaNICHT SPEZIFIZIERT
Arakawa, Kazuharu Institute for Advanced Biosciences, Keio University, Fujisawa, JapanNICHT SPEZIFIZIERT
Katayama, Toshiaki Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, JapanNICHT SPEZIFIZIERT
Kunieda, Takekazu Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, JapanNICHT SPEZIFIZIERT
Toyoda, Atsushi Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka, JapanNICHT SPEZIFIZIERT
Fujiyama, Asao Principles of Informatics Research Division, National Institute of Informatics, Tokyo, JapanNICHT SPEZIFIZIERT
Rothschild, Lynn J. Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and NASA Astrobiology Institute and Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USANICHT SPEZIFIZIERT
Datum:2013
Erschienen in:PLoS One
Referierte Publikation:Ja
In Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:8
DOI :10.1371/journal.pone.0064793
Verlag:PLOS
ISSN:1932-6203
Status:veröffentlicht
Stichwörter:UVC radiation, Tardigrades, extraordinary resistance, DNA repair
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:20 Jun 2013 08:15
Letzte Änderung:20 Jun 2013 08:15

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