elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Contact | Deutsch
Fontsize: [-] Text [+]

Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation

Horikawa, Daiki D. and Cumbers, John and Sakakibara, Iori and Rogoff, Dana and Leuko, Stefan and Harnoto, Raechel and Arakawa, Kazuharu and Katayama, Toshiaki and Kunieda, Takekazu and Toyoda, Atsushi and Fujiyama, Asao and Rothschild, Lynn J. (2013) Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation. PLoS One, 8 (6: e64793). PLOS. DOI: 10.1371/journal.pone.0064793. ISSN 1932-6203.

Full text not available from this repository.

Abstract

Tardigrades inhabiting terrestrial environments exhibit extraordinary resistance to ionizing radiation and UV radiation although little is known about the mechanisms underlying the resistance. We found that the terrestrial tardigrade Ramazzottius varieornatus is able to tolerate massive doses of UVC irradiation by both being protected from forming UVCinduced thymine dimers in DNA in a desiccated, anhydrobiotic state as well as repairing the dimers that do form in the hydrated animals. In R. varieornatus accumulation of thymine dimers in DNA induced by irradiation with 2.5 kJ/m² of UVC radiation disappeared 18 h after the exposure when the animals were exposed to fluorescent light but not in the dark. Much higher UV radiation tolerance was observed in desiccated anhydrobiotic R. varieornatus compared to hydrated specimens of this species. On the other hand, the freshwater tardigrade species Hypsibius dujardini that was used as control, showed much weaker tolerance to UVC radiation than R. varieornatus, and it did not contain a putative phrA gene sequence. The anhydrobiotes of R. varieornatus accumulated much less UVC-induced thymine dimers in DNA than hydrated one. It suggests that anhydrobiosis efficiently avoids DNA damage accumulation in R. varieornatus and confers better UV radiation tolerance on this species. Thus we propose that UV radiation tolerance in tardigrades is due to the both high capacities of DNA damage repair and DNA protection, a two-pronged survival strategy.

Document Type:Article
Title:Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation
Authors:
AuthorsInstitution or Email of Authors
Horikawa, Daiki D. Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and NASA Astrobiology Institute
Cumbers, John Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, United States of America
Sakakibara, Iori INSERM U1016, Institut Cochin, Paris, France and CNRS UMR 8104, Paris, France and Université Paris Descartes, Sorbonne Paris Cité , Paris, France
Rogoff, Dana Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America
Leuko, Stefan Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and German Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Radiation Biology Department, Linder Hoehe, Cologne (Koeln), Germany
Harnoto, Raechel California Polytechnic State University, San Luis Obispo, California, United States of America
Arakawa, Kazuharu Institute for Advanced Biosciences, Keio University, Fujisawa, Japan
Katayama, Toshiaki Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Kunieda, Takekazu Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan
Toyoda, Atsushi Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka, Japan
Fujiyama, Asao Principles of Informatics Research Division, National Institute of Informatics, Tokyo, Japan
Rothschild, Lynn J. Biospheric Science Branch, NASA Ames Research Center, Moffett Field, California, United States of America and NASA Astrobiology Institute and Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA
Date:2013
Journal or Publication Title:PLoS One
Refereed publication:Yes
In Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:8
DOI:10.1371/journal.pone.0064793
Publisher:PLOS
ISSN:1932-6203
Status:Published
Keywords:UVC radiation, Tardigrades, extraordinary resistance, DNA repair
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:20 Jun 2013 08:15
Last Modified:20 Jun 2013 08:15

Repository Staff Only: item control page

Browse
Search
Help & Contact
Informationen
electronic library is running on EPrints 3.3.12
Copyright © 2008-2012 German Aerospace Center (DLR). All rights reserved.