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Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans

Bauermeister, Anja and Hahn, Claudia and Rettberg, Petra and Reitz, Günther and Moeller, Ralf (2012) Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans. Archives of Microbiology, 194 (11), pp. 959-966. Springer-Verlag 2012. DOI: 10.1007/s00203-012-0834-x. ISSN 0302-8933.

Full text not available from this repository.

Abstract

To study the effects of heat shock on Deinococcus radiodurans and the role of DNA repair in high temperature resistance, different strains of D. radiodurans (wild type, recA, irrE, and pprA) were treated with temperatures ranging from 40 to 100 °C under wet and dry conditions. The mutant strains were more sensitive to wet heat of ≥60 °C and dry heat of ≥80 °C than the wild type. Both wild-type and DNA repair-deficient strains were much more resistant to high temperatures when exposed in the dried state as opposed to cells in suspension. Molecular staining techniques with the wild-type strain revealed that cells in the dried state were able to retain membrane integrity after drying and subsequent heat exposure, while heat-exposed cells in suspension showed significant loss of membrane integrity and respiration activity. The results suggest that the repair of DNA damage (e.g., DNA doublestrand breaks by RecA and PprA) is essential after treatment with wet heat at temperatures >60 °C and dry heat >80 °C, and the ability of D. radiodurans to stabilize its plasma membrane during dehydration might represent one aspect in the protection of dried cells from heat-induced membrane damage.

Document Type:Article
Title:Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans
Authors:
AuthorsInstitution or Email of Authors
Bauermeister, Anja Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Hahn, Claudia Saarland University, 66123 Saarbrücken, Germany
Rettberg, Petra Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Reitz, Günther Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Moeller, Ralf Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Date:2012
Journal or Publication Title:Archives of Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:194
DOI:10.1007/s00203-012-0834-x
Page Range:pp. 959-966
Publisher:Springer-Verlag 2012
ISSN:0302-8933
Status:Published
Keywords:Deinococcus radiodurans,  Heat resistance,  DNA damage,  Membrane lesions,  DNA repair mechanisms
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:05 Nov 2012 15:21
Last Modified:26 Mar 2013 13:43

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