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Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans

Bauermeister, Anja und Hahn, Claudia und Rettberg, Petra und Reitz, Günther und Moeller, Ralf (2012) Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans. Archives of Microbiology, 194 (11), Seiten 959-966. Springer-Verlag 2012. DOI: 10.1007/s00203-012-0834-x. ISSN 0302-8933.

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Kurzfassung

To study the effects of heat shock on Deinococcus radiodurans and the role of DNA repair in high temperature resistance, different strains of D. radiodurans (wild type, recA, irrE, and pprA) were treated with temperatures ranging from 40 to 100 °C under wet and dry conditions. The mutant strains were more sensitive to wet heat of ≥60 °C and dry heat of ≥80 °C than the wild type. Both wild-type and DNA repair-deficient strains were much more resistant to high temperatures when exposed in the dried state as opposed to cells in suspension. Molecular staining techniques with the wild-type strain revealed that cells in the dried state were able to retain membrane integrity after drying and subsequent heat exposure, while heat-exposed cells in suspension showed significant loss of membrane integrity and respiration activity. The results suggest that the repair of DNA damage (e.g., DNA doublestrand breaks by RecA and PprA) is essential after treatment with wet heat at temperatures >60 °C and dry heat >80 °C, and the ability of D. radiodurans to stabilize its plasma membrane during dehydration might represent one aspect in the protection of dried cells from heat-induced membrane damage.

Dokumentart:Zeitschriftenbeitrag
Titel:Roles of DNA repair and membrane integrity in heat resistance of Deinococcus radiodurans
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Bauermeister, Anja Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Hahn, Claudia Saarland University, 66123 Saarbrücken, Germany
Rettberg, Petra Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Reitz, Günther Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Moeller, Ralf Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Datum:2012
Erschienen in:Archives of Microbiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:194
DOI :10.1007/s00203-012-0834-x
Seitenbereich:Seiten 959-966
Verlag:Springer-Verlag 2012
ISSN:0302-8933
Status:veröffentlicht
Stichwörter:Deinococcus radiodurans,  Heat resistance,  DNA damage,  Membrane lesions,  DNA repair mechanisms
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:05 Nov 2012 15:21
Letzte Änderung:26 Mär 2013 13:43

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