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Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation

Mertens, Ramona (2012) Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation. Bachelor's, University of Applied Sciences Bonn-Rhein-Sieg.

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Abstract

The role of the two major DNA double strand break (DSB) repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), in Bacillus subtilis spore resistance to ionizing radiation had been investigated. Spores deficient in PNPase, HR- and NHEJ- mediated DNA repair were analyzed with regard to their survivability upon the exposure to X-rays and compared to the responses of B. subtilis BG214 wild-type spores. Spore resistance was observed to decrease in the following order (from the most resistant to the most sensitive ones): ΔrecQ ≥ wild-type > ΔrecN > ΔrecS > ΔrecJ > ΔpnpA > ΔrecU > ΔrecG > ΔrecO > ΔKu > ΔrecA > ΔrecF > ΔpnpA Ku > ΔrecR > ΔpnpA recA > ΔKu recA, indicating that HR and NHEJ both play a central role in protecting B. subtilis spores against potentially lethal environmental conditions. Further, it could be shown that ionizing radiation induces a significant time-delay in spore germination and subsequent outgrowth events, as demonstrated by germination profiling via well-plate-based germination assays as well as transcriptional profiling via cDNA microarray technology, suggesting the extensive expiration of repair of ionizing radiation-induced DNA damages during spore germination.

Document Type:Thesis (Bachelor's)
Title:Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation
Authors:
AuthorsInstitution or Email of Authors
Mertens, Ramona Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Date:2012
Number of Pages:106
Status:Published
Keywords:ionizing radiation, Bacillus subtilis spores, DNA double strand break (DSB) repair mechanisms
Institution:University of Applied Sciences Bonn-Rhein-Sieg
Department:Department of Natural Sciences
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:30 Oct 2012 09:39
Last Modified:30 Oct 2012 09:39

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