Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation
Mertens, Ramona (2012) Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation. Bachelor's, University of Applied Sciences Bonn-Rhein-Sieg.
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The role of the two major DNA double strand break (DSB) repair mechanisms, homologous recombination (HR) and non-homologous end-joining (NHEJ), in Bacillus subtilis spore resistance to ionizing radiation had been investigated. Spores deficient in PNPase, HR- and NHEJ- mediated DNA repair were analyzed with regard to their survivability upon the exposure to X-rays and compared to the responses of B. subtilis BG214 wild-type spores. Spore resistance was observed to decrease in the following order (from the most resistant to the most sensitive ones): ΔrecQ ≥ wild-type > ΔrecN > ΔrecS > ΔrecJ > ΔpnpA > ΔrecU > ΔrecG > ΔrecO > ΔKu > ΔrecA > ΔrecF > ΔpnpA Ku > ΔrecR > ΔpnpA recA > ΔKu recA, indicating that HR and NHEJ both play a central role in protecting B. subtilis spores against potentially lethal environmental conditions. Further, it could be shown that ionizing radiation induces a significant time-delay in spore germination and subsequent outgrowth events, as demonstrated by germination profiling via well-plate-based germination assays as well as transcriptional profiling via cDNA microarray technology, suggesting the extensive expiration of repair of ionizing radiation-induced DNA damages during spore germination.
|Document Type:||Thesis (Bachelor's)|
|Title:||Characterization of homologous recombination and non-homologous end-joining in DNA double-strand break repair of Bacillus subtilis spores after exposure to ionizing radiation|
|Number of Pages:||106|
|Keywords:||ionizing radiation, Bacillus subtilis spores, DNA double strand break (DSB) repair mechanisms|
|Institution:||University of Applied Sciences Bonn-Rhein-Sieg|
|Department:||Department of Natural Sciences|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Raumfahrt|
|HGF - Program Themes:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research area:||Raumfahrt|
|DLR - Program:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||R - Vorhaben Strahlenbiologie|
|Institutes and Institutions:||Institute of Aerospace Medicine > Radiation Biology|
|Deposited By:||Kerstin Kopp|
|Deposited On:||30 Oct 2012 09:39|
|Last Modified:||30 Oct 2012 09:39|
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