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Transcriptomic Responses of Germinating Bacillus subtilis Spores Exposed to 1.5 Years of Space and Simulated Martian Conditions on the EXPOSE-E Experiment PROTECT

Nicholson, Wayne L. und Moeller, Ralf und the PROTECT Team, * und Horneck, Gerda (2012) Transcriptomic Responses of Germinating Bacillus subtilis Spores Exposed to 1.5 Years of Space and Simulated Martian Conditions on the EXPOSE-E Experiment PROTECT. Astrobiology, 12 (5), Seiten 469-486. Mary Ann Liebert, Inc.. DOI: 10.1089/ast.2011.0748.

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Kurzfassung

Because of their ubiquity and resistance to spacecraft decontamination, bacterial spores are considered likely potential forward contaminants on robotic missions to Mars. Thus, it is important to understand their global responses to long-term exposure to space or martian environments. As part of the PROTECT experiment, spores of B. subtilis 168 were exposed to real space conditions and to simulated martian conditions for 559 days in low- Earth orbit mounted on the EXPOSE-E exposure platform outside the European Columbus module on the International Space Station. Upon return, spores were germinated, total RNA extracted, fluorescently labeled, and used to probe a custom Bacillus subtilis microarray to identify genes preferentially activated or repressed relative to ground control spores. Increased transcript levels were detected for a number of stress-related regulons responding to DNA damage (SOS response, SPβ prophage induction), protein damage (CtsR/Clp system), oxidative stress (PerR regulon), and cell envelope stress (SigV regulon). Spores exposed to space demonstrated a much broader and more severe stress response than spores exposed to simulated martian conditions. The results are discussed in the context of planetary protection for a hypothetical journey of potential forward contaminant spores from Earth to Mars and their subsequent residence on Mars.

Dokumentart:Zeitschriftenbeitrag
Titel:Transcriptomic Responses of Germinating Bacillus subtilis Spores Exposed to 1.5 Years of Space and Simulated Martian Conditions on the EXPOSE-E Experiment PROTECT
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Nicholson, Wayne L. Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Kennedy Space Center, Florida, USA.
Moeller, Ralf Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
the PROTECT Team, *NICHT SPEZIFIZIERT
Horneck, Gerda Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.
Datum:2012
Erschienen in:Astrobiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:12
DOI :10.1089/ast.2011.0748
Seitenbereich:Seiten 469-486
Verlag:Mary Ann Liebert, Inc.
Status:veröffentlicht
Stichwörter:Bacillus, Mars, Planetary protection, Spaceflight, Spores
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr, Verkehr und Weltraum (alt)
HGF - Programm:Raumfahrt, Weltraum (alt)
HGF - Programmthema:Forschung unter Weltraumbedingungen, W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Raumfahrt, Weltraum
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen, W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie, W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:08 Aug 2012 15:32
Letzte Änderung:20 Mär 2013 19:45

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