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Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms

Stieglmeier, Michaela und Rettberg, Petra und Barczyk, Simon und Bohmeier, Maria und Pukall, Rüdiger und Wirth, Reinhard und Moissl-Eichinger, Christine (2012) Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms. Astrobiology, 12 (6), Seiten 572-585. Mary Ann Liebert, Inc.. DOI: 10.1089/ast.2011.0735.

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Kurzfassung

The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA’s and ESA’s guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heatresistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods.

Dokumentart:Zeitschriftenbeitrag
Titel:Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Stieglmeier, Michaela Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany. Present address: Department of Genetics in Ecology, University of Vienna, Vienna, Austria.
Rettberg, Petra German Aerospace Center (DLR), Cologne, Germany.
Barczyk, Simon German Aerospace Center (DLR), Cologne, Germany.
Bohmeier, MariaGerman Aerospace Center (DLR), Cologne, Germany.
Pukall, Rüdiger Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
Wirth, Reinhard Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.
Moissl-Eichinger, Christine Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.
Datum:2012
Erschienen in:Astrobiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:12
DOI :10.1089/ast.2011.0735
Seitenbereich:Seiten 572-585
Verlag:Mary Ann Liebert, Inc.
Status:veröffentlicht
Stichwörter:Planetary protection, Clean rooms, Microbial diversity, Bioburden, qPCR
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr, Verkehr und Weltraum (alt)
HGF - Programm:Raumfahrt, Weltraum (alt)
HGF - Programmthema:Forschung unter Weltraumbedingungen, W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Raumfahrt, Weltraum
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen, W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie, W - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:08 Aug 2012 15:33
Letzte Änderung:26 Mär 2013 13:41

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