elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Contact | Deutsch
Fontsize: [-] Text [+]

Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms

Stieglmeier, Michaela and Rettberg, Petra and Barczyk, Simon and Bohmeier, Maria and Pukall, Rüdiger and Wirth, Reinhard and Moissl-Eichinger, Christine (2012) Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms. Astrobiology, 12 (6), pp. 572-585. Mary Ann Liebert, Inc.. DOI: 10.1089/ast.2011.0735.

Full text not available from this repository.

Abstract

The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA’s and ESA’s guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heatresistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods.

Document Type:Article
Title:Abundance and Diversity of Microbial Inhabitants in European Spacecraft-Associated Clean Rooms
Authors:
AuthorsInstitution or Email of Authors
Stieglmeier, Michaela Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany. Present address: Department of Genetics in Ecology, University of Vienna, Vienna, Austria.
Rettberg, Petra German Aerospace Center (DLR), Cologne, Germany.
Barczyk, Simon German Aerospace Center (DLR), Cologne, Germany.
Bohmeier, MariaGerman Aerospace Center (DLR), Cologne, Germany.
Pukall, Rüdiger Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
Wirth, Reinhard Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.
Moissl-Eichinger, Christine Institute of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.
Date:2012
Journal or Publication Title:Astrobiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:12
DOI:10.1089/ast.2011.0735
Page Range:pp. 572-585
Publisher:Mary Ann Liebert, Inc.
Status:Published
Keywords:Planetary protection, Clean rooms, Microbial diversity, Bioburden, qPCR
HGF - Research field:Aeronautics, Space and Transport, Aeronautics, Space and Transport (old)
HGF - Program:Space, Space (old)
HGF - Program Themes:Research under Space Conditions, W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Raumfahrt, Space
DLR - Program:R FR - Forschung unter Weltraumbedingungen, W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie, W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:08 Aug 2012 15:33
Last Modified:26 Mar 2013 13:41

Repository Staff Only: item control page

Browse
Search
Help & Contact
Informationen
electronic library is running on EPrints 3.3.12
Copyright © 2008-2012 German Aerospace Center (DLR). All rights reserved.