X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells
Hu, Yueyuan and Lau, Patrick and Baumstark-Khan, Christa and Hellweg, Christine E. and Reitz, Günther (2012) X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells. Advances in Space Research, 49 (10), pp. 1422-1431. Elsevier. DOI: 10.1016/j.asr.2012.02.001.
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To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21 (CDKN1A) was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and CDKN1A (p21) gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.
|Title:||X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells|
|Journal or Publication Title:||Advances in Space Research|
|In ISI Web of Science:||Yes|
|Page Range:||pp. 1422-1431|
|Keywords:||Space, Ionizing radiation, Osteoblastic differentiation, Gene expression analysis|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Raumfahrt|
|HGF - Program Themes:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research area:||Raumfahrt|
|DLR - Program:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||R - Vorhaben Strahlenbiologie|
|Institutes and Institutions:||Institute of Aerospace Medicine > Radiation Biology|
|Deposited By:||Kerstin Kopp|
|Deposited On:||26 Apr 2012 14:42|
|Last Modified:||20 Mar 2013 19:42|
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