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X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells

Hu, Yueyuan und Lau, Patrick und Baumstark-Khan, Christa und Hellweg, Christine E. und Reitz, Günther (2012) X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells. Advances in Space Research, 49 (10), Seiten 1422-1431. Elsevier. DOI: 10.1016/j.asr.2012.02.001.

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Kurzfassung

To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21 (CDKN1A) was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and CDKN1A (p21) gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.

Dokumentart:Zeitschriftenbeitrag
Titel:X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Hu, Yueyuan Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Lau, Patrick Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Baumstark-Khan, Christa Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Hellweg, Christine E.Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Reitz, Günther Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Datum:2012
Erschienen in:Advances in Space Research
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:49
DOI :10.1016/j.asr.2012.02.001
Seitenbereich:Seiten 1422-1431
Verlag:Elsevier
Status:veröffentlicht
Stichwörter:Space, Ionizing radiation, Osteoblastic differentiation, Gene expression analysis
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:26 Apr 2012 14:42
Letzte Änderung:20 Mär 2013 19:42

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