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X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells

Hu, Yueyuan and Lau, Patrick and Baumstark-Khan, Christa and Hellweg, Christine E. and Reitz, Günther (2012) X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells. Advances in Space Research, 49 (10), pp. 1422-1431. Elsevier. DOI: 10.1016/j.asr.2012.02.001.

Full text not available from this repository.

Abstract

To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex). In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix. Expression of mRNA encoding Runx2, transforming growth factor β1 (TGF-β1), osteocalcin (OCN), and p21 (CDKN1A) was analyzed. Exposure to IR reduced the growth rate and diminished cell survival of OCT-1 cells under standard conditions. Notably, calcium content analysis revealed that deposition of mineralized matrix increased significantly under osteogenic conditions after X-ray exposure in a time-dependent manner. In this study, higher radiation doses exert significant overall effects on TGF-β1, OCN, and CDKN1A (p21) gene expression, suggesting that gene expression following X-ray treatment is affected in a dose-dependent manner. Additionally, we verified that Runx2 was suppressed within 24 h after irradiation at 2 and 4 Gy. Although further studies are required to verify the molecular mechanism, our observations strongly suggest that treatment with IR markedly alters the differentiation and mineralization process of preosteoblastic cells.

Document Type:Article
Title:X-ray induced alterations in the differentiation and mineralization potential of murine preosteoblastic cells
Authors:
AuthorsInstitution or Email of Authors
Hu, Yueyuan Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Lau, Patrick Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Baumstark-Khan, Christa Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Hellweg, Christine E.Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Reitz, Günther Division of Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), Linder Hoehe, 51147 Koeln, Germany
Date:2012
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:49
DOI:10.1016/j.asr.2012.02.001
Page Range:pp. 1422-1431
Publisher:Elsevier
Status:Published
Keywords:Space, Ionizing radiation, Osteoblastic differentiation, Gene expression analysis
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:26 Apr 2012 14:42
Last Modified:20 Mar 2013 19:42

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