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Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine

Benardini, James N. and Vaishampayan, Parag A. and Schwendner, Petra and Swanner, Elizabeth and Fukui, Youhei and Osman, Sharif and Satomi, Masakata and Venkateswaran, Kasthuri (2011) Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine. International Journal of Systematic and Evolutionary Microbiology, 61 (6), 1338 -1343 . International Union of Microbiological Societies . DOI: 10.1099/ijs.0.021428-0.

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Abstract

A novel Gram-positive, motile, endospore-forming, aerobic bacterium was isolated from the NASA Phoenix Lander assembly clean room that exhibits 100% 16S rRNA gene sequence similarity to two strains isolated from a deep subsurface environment. All strains are rod-shaped, endosporeforming bacteria, whose endospores are resistant to UV radiation up to 500 J m-². A polyphasic taxonomic study including traditional phenotypic tests, fatty acid analysis, 16S rRNA gene sequencing and DNA–DNA hybridization analysis was performed to characterize these novel strains. The 16S rRNA gene sequencing convincingly grouped these novel strains within the genus Paenibacillus as a separate cluster from previously described species. The similarity of 16S rRNA gene sequences among the novel strains was identical but only 98.1 to 98.5% with their nearest neighbours Paenibacillus barengoltzii ATCC BAA-1209^T and Paenibacillus timonensis CIP 108005^T. The menaquinone MK-7 was dominant in these novel strains as shown in other species of the genus Paenibacillus. The DNA–DNA hybridization dissociation value was ,45% with the closest related species. The novel strains had DNA G+C contents of 51.9 to 52.8 mol%. Phenotypically, the novel strains can be readily differentiated from closely related species by the absence of urease and gelatinase and the production of acids from a variety of sugars including L-arabinose. The major fatty acid was anteiso-C(15 : 0) as seen in P. barengoltzii and P. timonensis whereas the proportion of C(16 : 0) was significantly different from the closely related species. Based on phylogenetic and phenotypic results, it was concluded that these strains represent a novel species of the genus Paenibacillus, for which the name Paenibacillus phoenicis sp. nov. is proposed. The type strain is 3PO2SA^T (5NRRL B-59348^T 5NBRC 106274^T).

Document Type:Article
Title:Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine
Authors:
AuthorsInstitution or Email of Authors
Benardini, James N. Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Vaishampayan, Parag A. Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Schwendner, Petra Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA and German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, 51147 Cologne, Germany
Swanner, ElizabethColorado Geological Sciences, University of Colorado, UCB 399, Boulder, CO 80309, USA
Fukui, YouheiNational Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan
Osman, Sharif Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Satomi, Masakata National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan
Venkateswaran, Kasthuri Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Date:2011
Journal or Publication Title:International Journal of Systematic and Evolutionary Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:61
DOI:10.1099/ijs.0.021428-0
Page Range:1338 -1343
Publisher:International Union of Microbiological Societies
Status:Published
Keywords:Paenibacillus phoenicis sp. nov., NASA Phoenix Lander assembly facility
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:22 Dec 2011 14:42
Last Modified:04 Apr 2013 16:34

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