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Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine

Benardini, James N. und Vaishampayan, Parag A. und Schwendner, Petra und Swanner, Elizabeth und Fukui, Youhei und Osman, Sharif und Satomi, Masakata und Venkateswaran, Kasthuri (2011) Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine. International Journal of Systematic and Evolutionary Microbiology, 61 (6), . International Union of Microbiological Societies . DOI: 10.1099/ijs.0.021428-0.

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Kurzfassung

A novel Gram-positive, motile, endospore-forming, aerobic bacterium was isolated from the NASA Phoenix Lander assembly clean room that exhibits 100% 16S rRNA gene sequence similarity to two strains isolated from a deep subsurface environment. All strains are rod-shaped, endosporeforming bacteria, whose endospores are resistant to UV radiation up to 500 J m-². A polyphasic taxonomic study including traditional phenotypic tests, fatty acid analysis, 16S rRNA gene sequencing and DNA–DNA hybridization analysis was performed to characterize these novel strains. The 16S rRNA gene sequencing convincingly grouped these novel strains within the genus Paenibacillus as a separate cluster from previously described species. The similarity of 16S rRNA gene sequences among the novel strains was identical but only 98.1 to 98.5% with their nearest neighbours Paenibacillus barengoltzii ATCC BAA-1209^T and Paenibacillus timonensis CIP 108005^T. The menaquinone MK-7 was dominant in these novel strains as shown in other species of the genus Paenibacillus. The DNA–DNA hybridization dissociation value was ,45% with the closest related species. The novel strains had DNA G+C contents of 51.9 to 52.8 mol%. Phenotypically, the novel strains can be readily differentiated from closely related species by the absence of urease and gelatinase and the production of acids from a variety of sugars including L-arabinose. The major fatty acid was anteiso-C(15 : 0) as seen in P. barengoltzii and P. timonensis whereas the proportion of C(16 : 0) was significantly different from the closely related species. Based on phylogenetic and phenotypic results, it was concluded that these strains represent a novel species of the genus Paenibacillus, for which the name Paenibacillus phoenicis sp. nov. is proposed. The type strain is 3PO2SA^T (5NRRL B-59348^T 5NBRC 106274^T).

Dokumentart:Zeitschriftenbeitrag
Titel:Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Benardini, James N. Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Vaishampayan, Parag A. Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Schwendner, Petra Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA and German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, 51147 Cologne, Germany
Swanner, ElizabethColorado Geological Sciences, University of Colorado, UCB 399, Boulder, CO 80309, USA
Fukui, YouheiNational Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan
Osman, Sharif Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Satomi, Masakata National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan
Venkateswaran, Kasthuri Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA
Datum:2011
Erschienen in:International Journal of Systematic and Evolutionary Microbiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:61
DOI :10.1099/ijs.0.021428-0
Seitenbereich:
Verlag:International Union of Microbiological Societies
Status:veröffentlicht
Stichwörter:Paenibacillus phoenicis sp. nov., NASA Phoenix Lander assembly facility
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:22 Dez 2011 14:42
Letzte Änderung:04 Apr 2013 16:34

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