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Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity – Description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room

Behrendt, Undine and Schumann, Peter and Stieglmeier, Michaela and Pukall, Rüdiger and Augustin, Jürgen and Spröer, Cathrin and Schwendner, Petra and Moissl-Eichinger, Christine and Ulrich, Andreas (2011) Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity – Description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room. Systematic and Applied Microbiology, 33 (6), pp. 328-336. Elsevier. DOI: 10.1016/j.syapm.2010.07.004.

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Abstract

In the course of studying the influence of N-fertilization on N₂ and N₂O flux rates in relation to soil bacterial community composition of a long-term fertilization experiment in fen peat grassland, a strain group was isolated that was related to a strain isolated from a spacecraft assembly clean room during diversity studies of microorganisms, which withstood cleaning and bioburden reduction strategies. Both the fen soil isolates and the clean room strain revealed versatile physiological capacities in N-transformation processes by performing heterotrophic nitrification, respiratory ammonification and denitrification activity. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the investigated isolates belonged to the genus Paenibacillus. Sequence similarities lower than 97% in comparison to established species indicated a separate species position. Except for the peptidoglycan type (A4alpha L-Lys–D-Asp), chemotaxonomic features of the isolates matched the genus description, but differences in several physiological characteristics separated them from related species and supported their novel species status. Despite a high 16S rRNA gene sequence similarity between the clean room isolate ES_MS17^T and the representative fen soil isolate N3/975^T, DNA–DNA hybridization studies revealed genetic differences at the species level. These differences were substantiated by MALDI-TOF MS analysis, ribotyping and several distinct physiological characteristics. On the basis of these results, it was concluded that the fen soil isolates and the clean room isolate ES_MS17^T represented two novel species for which the names Paenibacillus uliginis sp. nov. (type strain N3/975^T = DSM 21861^T = LMG 24790^T) and Paenibacillus purispatii sp. nov. (type strain ES_MS17^T = DSM 22991^T = CIP 110057^T) are proposed.

Document Type:Article
Title:Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity – Description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room
Authors:
AuthorsInstitution or Email of Authors
Behrendt, Undine Leibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany
Schumann, Peter DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany
Stieglmeier, Michaela University of Regensburg, Institute for Microbiology and Archea Center, Universitätsstr. 31, D-93053 Regensburg, Germany
Pukall, Rüdiger DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany
Augustin, JürgenLeibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany
Spröer, Cathrin DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany
Schwendner, Petra University of Regensburg, Institute for Microbiology and Archea Center, Universitätsstr. 31, D-93053 Regensburg, Germany and German Aerospace Center, Institute of Aerospace Medicine, Linder Höhe, 51147 Cologne, Germany
Moissl-Eichinger, Christine University of Regensburg, Institute for Microbiology and Archea Center, Universitätsstr. 31, D-93053 Regensburg, Germany
Ulrich, Andreas Leibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany
Date:2011
Journal or Publication Title:Systematic and Applied Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:33
DOI:10.1016/j.syapm.2010.07.004
Page Range:pp. 328-336
Publisher:Elsevier
Status:Published
Keywords:Paenibacillus uliginis sp. nov., Paenibacillus purispatii sp. nov., Heterotrophic nitrification, Denitrification, Dissimilatory nitrate/nitrite reduction to ammonium, Space craft associated clean rooms, Planetary protection
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:22 Dec 2011 14:43
Last Modified:04 Apr 2013 16:34

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