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Recovery of Bacillus Spore Contaminants from Rough Surfaces: a Challenge to Space Mission Cleanliness Control

Probst, Alexander und Facius, Rainer und Wirth, Reinhard und Wolf, Marco und Moissl-Eichinger, Christine (2011) Recovery of Bacillus Spore Contaminants from Rough Surfaces: a Challenge to Space Mission Cleanliness Control. Applied and Environmental Microbiology, 77 (5), Seiten 1628-1637. American Society for Microbiology. DOI: 10.1128/AEM.02037-10.

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Kurzfassung

Microbial contaminants on spacecraft can threaten the scientific integrity of space missions due to probable interference with life detection experiments. Therefore, space agencies measure the cultivable spore load (“bioburden”) of a spacecraft. A recent study has reported an insufficient recovery of Bacillus atrophaeus spores from Vectran fabric, a typical spacecraft airbag material (A. Probst, R. Facius, R. Wirth, and C. Moissl- Eichinger, Appl. Environ. Microbiol. 76:5148-5158, 2010). Here, 10 different sampling methods were compared for B. atrophaeus spore recovery from this rough textile, revealing significantly different efficiencies (0.5 to 15.4%). The most efficient method, based on the wipe-rinse technique (foam-spatula protocol; 13.2% efficiency), was then compared to the current European Space Agency (ESA) standard wipe assay in sampling four different kinds of spacecraft-related surfaces. Results indicate that the novel protocol out-performed the standard method with an average efficiency of 41.1% compared to 13.9% for the standard method. Additional experiments were performed by sampling Vectran fabric seeded with seven different spore concentrations and five different Bacillus species (B. atrophaeus, B. anthracis Sterne, B. megaterium, B. thuringiensis, and B. safensis). Among these, B. atrophaeus spores were recovered with the highest (13.2%) efficiency and B. anthracis Sterne spores were recovered with the lowest (0.3%) efficiency. Different inoculation methods of seeding spores on test surfaces (spotting and aerosolization) resulted in different spore recovery efficiencies. The results of this study provide a step forward in understanding the spore distribution on and recovery from rough surfaces. The results presented will contribute relevant knowledge to the fields of astrobiology and B. anthracis research.

Dokumentart:Zeitschriftenbeitrag
Titel:Recovery of Bacillus Spore Contaminants from Rough Surfaces: a Challenge to Space Mission Cleanliness Control
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Probst, Alexander Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany and Lander Systems and Space Robotics, Astrium Space Transportation, Airbus Allee 1, 28199 Bremen, Germany
Facius, Rainer German Aerospace Center, Linder Hoehe, 51147 Cologne, Germany
Wirth, Reinhard Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Wolf, MarcoLander Systems and Space Robotics, Astrium Space Transportation, Airbus Allee 1, 28199 Bremen, Germany
Moissl-Eichinger, Christine Institute of Microbiology and Archaea Center, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
Datum:2011
Erschienen in:Applied and Environmental Microbiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:77
DOI :10.1128/AEM.02037-10
Seitenbereich:Seiten 1628-1637
Verlag:American Society for Microbiology
Status:veröffentlicht
Stichwörter:B. anthracis, microbial contaminants on spacecraft, cultivable spore load (“bioburden”) of a spacecraft, European Space Agency (ESA) standard wipe assay, Astrobiology
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kerstin Kopp
Hinterlegt am:15 Dez 2011 14:21
Letzte Änderung:04 Apr 2013 16:33

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