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Effects of alfacalcidol on circulating cytokines and growth factors in rat skeletal muscle

Testerink, Janwillem and Jaspers, Richard and Rittweger, Jörn and de Haan, Arnold and Degens, Hans (2011) Effects of alfacalcidol on circulating cytokines and growth factors in rat skeletal muscle. Journal of Physiological Sciences, 61, pp. 525-535. ISSN 1880-6546.

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Abstract Supra-physiological levels of vitamin D induce skeletal muscle atrophy, which may be particularly detrimental in already sarcopaenic elderly. Neither the cause nor whether the atrophy is fibre type specific are known. To obtain supraphysiological levels of circulating vitamin D (1,25(OH)2D3) 27.5-month-old female Fischer344 9 Brown Norway F1 rats were orally treated for 6 weeks with vehicle or the vitamin D analogue alfacalcidol. Alfacalcidol treatment induced a 22% decrease in body mass and 17% muscle atrophy. Fibre atrophy was restricted to type IIb fibres in the low-oxidative part of the gastrocnemius medialis only (-22%; P\0.05). There was a concomitant 1.6-fold increase in mRNA expression of the ubiquitin ligase MuRF-1 (P\0.001), whereas those of insulin-like growth factor 1 and myostatin were not affected. Circulating IL-6 was unaltered, but leptin and adiponectin were decreased (-39%) and increased (64%), respectively. The treated rats also exhibited a reduced food intake. In conclusion, supraphysiological levels of circulating 1,25(OH)2D3 cause preferential atrophy of type IIb fibres, which is associated with an increased expression of MuRF- 1 without evidence of systemic inflammation. The atrophy and loss of body mass in the presence of supra-physiological levels of vitamin D are primarily due to a reduced food intake.

Document Type:Article
Title:Effects of alfacalcidol on circulating cytokines and growth factors in rat skeletal muscle
AuthorsInstitution or Email of Authors
Testerink, JanwillemFree University Amsterdam
Jaspers, RichardFree University Amsterdam
Rittweger, Jörnjoern.rittweger@dlr.de
de Haan, ArnoldFree University Amsterdam
Degens, HansManchester Metropolitan University
Journal or Publication Title:Journal of Physiological Sciences
Refereed publication:No
In ISI Web of Science:Yes
Page Range:pp. 525-535
Keywords:Vitamin D, Atrophy, IGF-1, Myostatin, Aging, E3-ligase
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Integrative Studien (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Space Physiology
Deposited By: Jörn Rittweger
Deposited On:06 Dec 2011 12:24
Last Modified:12 Dec 2013 21:27

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