Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation
Beblo, Kristina and Douki, Thierry and Schmalz, Gottfried and Rachel, Reinhard and Wirth, Reinhard and Huber, Harald and Reitz, Guenther and Rettberg, Petra (2011) Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation. Archives of Microbiology, 193 (11), pp. 797-809. Springer. DOI: 10.1007/s00203-011-0718-5. ISSN 0302-8933.
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In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylogenetically deep-branching thermophilic Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions. If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic microorganisms.
|Title:||Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation|
|Journal or Publication Title:||Archives of Microbiology|
|In ISI Web of Science:||Yes|
|Page Range:||pp. 797-809|
|Keywords:||Radiation, UV-C, Ionizing, Survival, Hyperthermophilic, Desiccation|
|HGF - Research field:||Aeronautics, Space and Transport|
|HGF - Program:||Raumfahrt|
|HGF - Program Themes:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research area:||Raumfahrt|
|DLR - Program:||R FR - Forschung unter Weltraumbedingungen|
|DLR - Research theme (Project):||R - Vorhaben Strahlenbiologie|
|Institutes and Institutions:||Institute of Aerospace Medicine > Radiation Biology|
|Deposited By:||Kerstin Kopp|
|Deposited On:||11 Oct 2011 10:33|
|Last Modified:||07 Feb 2013 20:09|
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