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Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation

Beblo, Kristina and Douki, Thierry and Schmalz, Gottfried and Rachel, Reinhard and Wirth, Reinhard and Huber, Harald and Reitz, Guenther and Rettberg, Petra (2011) Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation. Archives of Microbiology, 193 (11), pp. 797-809. Springer. DOI: 10.1007/s00203-011-0718-5. ISSN 0302-8933.

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Abstract

In this study, we investigated the ability of several (hyper-) thermophilic Archaea and phylogenetically deep-branching thermophilic Bacteria to survive high fluences of monochromatic UV-C (254 nm) and high doses of ionizing radiation, respectively. Nine out of fourteen tested microorganisms showed a surprisingly high tolerance against ionizing radiation, and two species (Aquifex pyrophilus and Ignicoccus hospitalis) were even able to survive 20 kGy. Therefore, these species had a comparable survivability after exposure to ionizing radiation such as Deinococcus radiodurans. In contrast, there was nearly no difference in survival of the tested strains after exposure to UV-C under anoxic conditions. If the cells had been dried in advance of UV-C irradiation, they were more sensitive to UV-C radiation compared with cells irradiated in liquid suspension; this effect could be reversed by the addition of protective material like sulfidic ores before irradiation. By exposure to UV-C, photoproducts were formed in the DNA of irradiated Archaea and Bacteria. The distribution of the main photoproducts was species specific, but the amount of the photoproducts was only partly dependent on the applied fluence. Overall, our results show that tolerance to radiation seems to be a common phenomenon among thermophilic and hyperthermophilic microorganisms.

Document Type:Article
Title:Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation
Authors:
AuthorsInstitution or Email of Authors
Beblo, Kristina Radiation Biology Division, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Linder Hoehe, 51147 Cologne, Germany and Institute for Microbiology and Archaea Center, Faculty of Biology and Preclinical Medicine, University Regensburg
Douki, Thierry Laboratoire ‘‘Lésions des Acides Nucléiques’’, Service de Chimie Inorganique et Biologique CEA/DSM/Département de Recherche Fondamentale sur la Matiére Condensée, CEA-Grenoble, Grenoble, France
Schmalz, Gottfried Department of Operative Dentistry and Periodontology, Regensburg University Clinics, Regensburg, Germany
Rachel, Reinhard Center for Electron Microscopy, Faculty of Biology and Preclinical Medicine, University Regensburg, Regensburg, Germany
Wirth, Reinhard Institute for Microbiology and Archaea Center, Faculty of Biology and Preclinical Medicine, University Regensburg, Regensburg, Germany
Huber, HaraldInstitute for Microbiology and Archaea Center, Faculty of Biology and Preclinical Medicine, University Regensburg, Regensburg, Germany
Reitz, Guenther Radiation Biology Division, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Linder Hoehe, 51147 Cologne, Germany
Rettberg, Petra Radiation Biology Division, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Linder Hoehe, 51147 Cologne, Germany
Date:2011
Journal or Publication Title:Archives of Microbiology
Refereed publication:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:193
DOI:10.1007/s00203-011-0718-5
Page Range:pp. 797-809
Publisher:Springer
ISSN:0302-8933
Status:Published
Keywords:Radiation, UV-C, Ionizing, Survival, Hyperthermophilic, Desiccation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kerstin Kopp
Deposited On:11 Oct 2011 10:33
Last Modified:07 Feb 2013 20:09

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