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Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach

Agostini, Francesco und Dalla Libera, Luciano und Rittweger, Jörn und Mazzucco, Sara und Jurdana, Mihaela und Mekjavic, Igor und Pisot, Rado und Gorza, Luisa und Narici, Marco und Biolo, Gianni (2010) Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach. The Journal of Physiology, 588 (24), Seiten 5089-5104. ISSN 0022-3751.

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Kurzfassung

Oxidative stress is often associated to inactivity-mediated skeletal muscle atrophy. Glutathione is one of the major antioxidant systems stimulated, both at muscular and systemic level, by activation of oxidative processes. We measured changes in glutathione availability, oxidative stress induction and the extent of atrophy mediated by 35 days of experimental bed rest in vastus lateralis muscle of healthy human volunteers. To assess muscle glutathione synthesis, we applied a novel single-biopsy and double-tracer ([2H2]glycine and [15N]glycine) approach based on evaluation of steady-state precursor incorporation in product. The correlations between the traditional (multiple-samples, one-tracer) and new (one-sample, double-tracer infusion) methods were analysed in erythrocytes by Passing–Bablok and Altman–Bland tests. Muscle glutathione absolute synthesis rate increased following bed rest from 5.5±1.1 to 11.0±1.5 mmol (kg wet tissue)−1 day−1 (mean ± s.e.m.; n =9; P =0.02) while glutathione concentration failed to change significantly. Bed rest induced vastus lateralis muscle atrophy, as assessed by pennation angle changes measured by ultrasonography (from 18.6±1.0 to 15.3±0.9 deg; P =0.01) and thickness changes (from 2.3±0.2 to 1.9±0.1 cm; P <0.001). Moreover, bed rest increased protein oxidative stress, as measured by muscle protein carbonylation changes (from 0.6±0.1 to 1.00±0.1 Oxydized-to-total protein ratio; P <0.04). In conclusion, we developed in erythrocytes a new minimally invasive method to determine peptide synthesis rate in human tissues. Application of the new method to skeletal muscle suggests that disuse atrophy is associated to oxidative stress induction aswell as to compensatory activation of the glutathione system.

Dokumentart:Zeitschriftenbeitrag
Titel:Effects of inactivity on human muscle glutathione synthesis by a double-tracer and single-biopsy approach
Autoren:
AutorenInstitution oder E-Mail-Adresse der Autoren
Agostini, FrancescoUniversity of Trieste
Dalla Libera, LucianoUniversity of Padova
Rittweger, Jörnjoern.rittweger@dlr.de
Mazzucco, SaraUniversity of Trieste
Jurdana, MihaelaUniversity of Primorska
Mekjavic, IgorJozef Stefan Institute
Pisot, RadoUniversity of Primorska
Gorza, LuisaUniversity of Padova
Narici, MarcoManchester Metropolitan University
Biolo, GianniUniversity of Trieste
Datum:2010
Erschienen in:The Journal of Physiology
Referierte Publikation:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:588
Seitenbereich:Seiten 5089-5104
ISSN:0022-3751
Status:veröffentlicht
Stichwörter:Bed rest, muscle atrophy
HGF - Forschungsbereich:Verkehr und Weltraum (alt)
HGF - Programm:Weltraum (alt)
HGF - Programmthema:W FR - Forschung unter Weltraumbedingungen (alt)
DLR - Schwerpunkt:Weltraum
DLR - Forschungsgebiet:W FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):W - Vorhaben Integrative Studien (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Weltraumphysiologie
Hinterlegt von: Jörn Rittweger
Hinterlegt am:09 Feb 2011 11:01
Letzte Änderung:12 Dez 2013 21:13

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